DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D | ko |
dc.contributor.author | Park, DH | ko |
dc.contributor.author | Kang, NG | ko |
dc.contributor.author | Namkoong, Y | ko |
dc.contributor.author | Shin, HS | ko |
dc.date.accessioned | 2021-02-22T08:50:10Z | - |
dc.date.available | 2021-02-22T08:50:10Z | - |
dc.date.created | 2020-10-14 | - |
dc.date.issued | 1996-10 | - |
dc.identifier.citation | MOLECULES AND CELLS, v.6, no.5, pp.577 - 581 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://hdl.handle.net/10203/280966 | - |
dc.description.abstract | A new embryonic stem (ES) cell line, H3, has been established from a 3.5-day p.c. blastocyst of the 129/sv strain of mice. The degree of germ-line competence of this cell line was compared with that of J1 sublines in the FvB/N host background, an albino inbred strain which is preferably used in the generation of transgenic mice via pronucleus injection. All chimeras of the FvB/N background derived from H3 or J1 cells showed a low degree coat color chimerism. However, contrary to the previous report on the germ-line incompatibity of the FvB/N strain with 129-derived ES cells, H3 cells gave germ line transmissions in five out of six FvB/N based chimeras. This property of H3 cells contrasted with those of J1 cells which did not give any significant germ line transmission from chimeras made in the same FvB/N host whereas they were efficient in forming a germ-line chimerism in the C 57BL/6J background. These results conform to the previous observations by others that the germ-line compatibility of ES cells with a host strain is dependent on clonal lines. FvB/N mice are known to be a robust breeder and the females respond well to hormone-induced superovulation yielding a large number of germ-line compatible blastocysts. Such advantages of FvB/N may be utilized in the study of gene targeting when this host is combined with H3 ES cells, and will results in saving costs and efforts required for such experiments. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | A new embryonic stem cell line with germ-line competence in the FvB/N background | - |
dc.type | Article | - |
dc.identifier.wosid | A1996VQ07000011 | - |
dc.identifier.scopusid | 2-s2.0-0642304048 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 577 | - |
dc.citation.endingpage | 581 | - |
dc.citation.publicationname | MOLECULES AND CELLS | - |
dc.contributor.localauthor | Kim, D | - |
dc.contributor.nonIdAuthor | Park, DH | - |
dc.contributor.nonIdAuthor | Kang, NG | - |
dc.contributor.nonIdAuthor | Namkoong, Y | - |
dc.contributor.nonIdAuthor | Shin, HS | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MOUSE EMBRYO | - |
dc.subject.keywordPlus | EGF RECEPTOR | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | DERIVATION | - |
dc.subject.keywordPlus | DISRUPTION | - |
dc.subject.keywordPlus | MUTATION | - |
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