Development of a genome swapping method and its application for hybrid microorganisms = 미생물 유전체 치환기술 개발 및 이를 이용한 하이브리드 균주 구축

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Based on whole-genome sequences, strain reconstruction studies for improved industrial applications have been initiated. For the construction of a custom-designed microorganism, an efficient genetic engineering tool which can transfer large genomic segments carrying useful genomic information is needed. We developed a novel and efficient method called genome swapping using Cre/lox site-specific recombination and a γ-ori replication system that can transfer large genomic segments between various microorganisms. In this study, we attempted to construct an IS-free Escherichia coli B strain, which is a popular industrial host strain in the production of useful biomaterials. However, mobile DNA elements such as insertion sequence elements (IS) and transposases caused a genetic unstableness. To eliminate this problem, we targeted IS elements for removal in 5 target regions and deleted IS elements within these target regions. The genome swapping method may be used to transfer IS-deleted target regions of the E. coli chromosome in one cell for the rapid construction of an IS-free E. coli B strain. Also, it is possible to construct a minimized genome and a hybrid microorganism for use in bioindustry and biotechnology.
Advisors
Kim, Sun-Changresearcher김선창researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
264267/325007  / 020053378
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ iv, 35 p. ]

Keywords

Cre/lox site specific recombination; large fragment; genome swapping; hybrid; 대장균 B 균주; 하이브리드; Cre/lox 재조합; 유전체 치환기술; E.coli B Strain

URI
http://hdl.handle.net/10203/28089
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=264267&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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