Measurement of trinucleotide repeat instability in yeast = 효모의 삼핵산 반복서열의 불안정성 측정에 관한 연구

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Instability of trinucleotide repeats (TNRs) is responsible for at least 15 hereditary disorders in humans. There is substantial evidence that alterations of the number of these repeats are due to the formation of secondary structures such as hairpins or slipped intermediates during lagging-strand DNA replication. Saccharomyces cerevisiae (S. cerevisiae) Dna2, which possesses endonuclease and helicase activities, plays a critical role in Okazaki fragment processing. The N-terminal 45-kDa domain of Dna2 binds to hairpin structures, targeting the enzyme specifically to a secondary-structured flap DNA. This Dna2-associated activity is required for the endonucleolytic removal of hairpin flaps by Dna2 with the aid of its helicase activity. Thus, the efficient removal of flaps, generated during the replication of lagging strand templates containing TNRs, requires the coordinated action of all three activities of Dna2. Here we show in vivo that Dna2 endonuclease/helicase is required for the stable maintenance of TNRs in S. cerevisiae. Our results demonstrate that the malfunction of Dna2 during Okazaki fragment processing in eukaryotes contributes to TNR instability.
Advisors
Seo, Yeon-Sooresearcher서연수researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
264262/325007  / 020053018
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ iv, 45 p. ]

Keywords

Trinucleotide repeat; TNR; 삼핵산 반복서열; Dna2

URI
http://hdl.handle.net/10203/28084
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=264262&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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