DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Tae-Gwan | - |
dc.contributor.advisor | 박태관 | - |
dc.contributor.author | Oh, In-Kyung | - |
dc.contributor.author | 오인경 | - |
dc.date.accessioned | 2011-12-12T08:54:37Z | - |
dc.date.available | 2011-12-12T08:54:37Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255233&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28070 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2006.2, [ ⅴ, 40 p. ] | - |
dc.description.abstract | The surface of adenovirus (ADV) was modified with folate-poly(ethylene glycol) (FOL-PEG) conjugate to overcome the inherent problems associated with viral gene therapy. The ADV conjugated with FOL moieties at the distal end of PEG chains could not only reduce immune response, but also be retargeted to folate receptor overexpressing tumor cells. ADV coding the green fluorescent protein (GFP) gene was used to immobilize PEG or FOL-PEG conjugate on the surface to comparatively evaluate their extents of retargeting and immune response, as compared to those of naked ADV. The ADV conjugated with FOL-PEG exhibited a greatly enhanced level of GFP expression than naked or PEG immobilized ADV in a folate receptor overexpressing cell line (KB cells), but not in a folate receptor deficient cell line (A549 cells), suggesting that the retargeting of ADV could be achieved by immobilizing FOL-PEG conjugate. ADV immobilized with PEG or FOL-PEG also significantly lowered innate immune response, as judged from determining the amount of interleukin 6 released from macrophage. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Retargeting | - |
dc.subject | PEG | - |
dc.subject | Folate | - |
dc.subject | Adenovirus | - |
dc.subject | Tumor | - |
dc.subject | 유전자 치료 | - |
dc.subject | 암세포 | - |
dc.subject | PEG | - |
dc.subject | Folate | - |
dc.subject | 아데노바이러스 | - |
dc.title | Folate immobilized and PEGylated adenovirus for retargeting to tumor cells | - |
dc.title.alternative | Folate-PEG 접합 아데노바이러스를 이용한 암세포의 유전자 치료에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 255233/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020043333 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.contributor.localauthor | 박태관 | - |
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