Polyphasic taxonomy of microbacterium soli US15, a novel xylan-degrading bacterium isolated from the soil in Ulsan, Korea = 색소 기질법을 이용하여 울산 토양에서 분리한 새로운 자일란 분해균, Microbacterium soli US15 의 분류학적 연구

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A novel xylan-degrading bacterium $US15^T$ was isolated from soil near Ulsan metallic treatement plant. The strain was Gram-positive, non-motile, non-spore-forming and coccoid-shaped rod. It formed yellow colonies on nutrient agar and showed chemotaxonomic markers that were consistent with classification in the genus Microbacterium, i.e. MK-12, MK-11 and MK-13 as major menaquinones; predominating iso- and anteiso-branched cellular fatty acids; glucose, galactose, fucose and mannose as cell-wall sugars; peptidoglycan-type B2β with glycolyl residues; G+C content of 66.5 %. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed strain $US15^T$ was most similar to M. imperiale (96.9%), M. arborescens (96.8%) and M. ulmi (96.2%). It is clear from phylogenetic analyses based on complete 16S rRNA sequences data that the isolate is a member of genus Microbacterium and represents a differentiated phyletic line that can be equated to a separate genomic species. On the basis of these genotypic and phenotypic results, it is proposed that $US15^T ( = DSM 16915^T = KCTC 19080^T)$ be classified as the type strain of a novel Microbacterium species, for which the names Microbacterium soli sp. nov. is proposed.
Advisors
Lee, Sung-Taikresearcher이성택researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
249536/325007  / 020033921
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.8, [ v, 40 p. ]

Keywords

Polyphasic taxonomy; 세균 분류학

URI
http://hdl.handle.net/10203/28062
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=249536&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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