Down-regulation of cold inducible RNA-binding protein using siRNA does not overcome hypothermic growth arrest = siRNA 를 이용한 cold inducible RNA-binding protein 의 발현 억제가 재조합 CHO 세포의 저온배양에 미치는 영향에 관한 연구

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When cells are cultured in low temperature (32°C), cold inducible RNA-binding protein (cirp) is highly expressed, which is known to suppress cell growth in low temperature. In low temperature, specific productivity (qp) of recombinant CHO (rCHO) cells producing foreign protein is increased and the quality of foreign protein is enhanced against normal temperature (37°C). But lowed specific growth rate of rCHO cells is an obstable to high production of therapeutic proteins. We cloned CHO cirp cDNA and vector based siRNAs against cirp down-regulate cirp stably both mRNA level and protein level. From the experiments of these cirp down-regulated clones, we could find specific growth rates of cirp down-regulated clones are not recovered in compared with control in low temperature culture. And, specific growth rate of the clones with highly expresed level of cirp was not reduced in compared with that of clones with normaly expressed level of cirp. Specific productivity of cells was relatively constant regardless of cirp expressioin levels, too. Cell cycle distributions of cells of which cirp is down-regulated in low temperature are similar to control, and cirp over-expressed clones showed the same results, too. So we concluded that in CHO cells, down-regulation of only cirp is not sufficient to recover growth arrest in low temperature culture.
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
243534/325007  / 020033682
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ v, 49 p. ]

Keywords

Sialyltransferase; CHOse; low temperature; siRNA; Cold inducible RNA binding protein (cirp); DEAE Chromatography; DEAE 크로마토그래피; 세포유도사멸 시알산 전이효소; 재조합 CHO 세포Rev-Caspase-3; 저온배양; siRNA; Cirp; 2-D HPLCnjugation

URI
http://hdl.handle.net/10203/28059
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243534&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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