(A) role of drosophila spitz gene in the output pathway of circadian rhythms = 일주성 생체 리듬의 전달 경로에서의 초파리 spitz 유전자의 기능 연구

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A rhythm with 24 hour period is generated by a circadian pacemaker. This controls daily rhythms in behavior and physiology and sustains even in the absence of environmental time cues. The circadian clock consists of three parts, input signal, central clock oscillator, and output signal. Drosophila clock pacemaker is located in lateral neurons of brain. The molecular components of the circadian pacemaker form a transcription based negative feedback loop using positive and negative elements, respectively clock/cycle and period/timeless. And cryptochrome is an input clock gene, a major photoreceptor for behavioral circadian rhythms. Output signal from the circadian clock controls rhythmic behavior poorly understood. The signaling molecules that mediate output from pacemaker to behavior are not known, although a secreted neuropeptide, pigment dispersing factor, may be a crucial output element in Drosophila. Here, we identified a novel circadian mutant, spitz, and is a secreted ligand that can be a candidate for output signaling molecule. Hypomorphic mutations of spitz (spi) gene produced abnormal circadian rhythms in locomotor activity. Hypomorph showed normal expression of clock gene, period, but weakened especially in Lateral Neurons (LNs), circadian pacemaker. And oscillation of phospho mitogen-activated protein kinase (p-MAPK) was strongly dampened in spitz hypomorphs. And spitz was expressed broadly in larval brain including LNs and DNs. All these suggest that spitz gene can regulate output signaling pathways
Advisors
Choe, Joon-Horesearcher최준호researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
243533/325007  / 020033959
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ v, 47 p. ]

Keywords

Sialyltransferase; Apoptosisrase; Rev-Caspase-3; Circadian Rhythm Outputediated endocytosis; DEAE Chromatography; DEAE 크로마토그래피; 세포유도사멸 시알산 전이효소; Rev-Caspase-3; 유도 세포내 전달; 생체 리듬 전달; 2-D HPLCnjugation

URI
http://hdl.handle.net/10203/28058
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243533&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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