Chemical genomic studies of small molecule modulator of Wnt/β-catenin pathway = Wnt/β -catenin pathway의 저분자 화합물 조절인자에 관한 화학유전체학적 연구

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dc.contributor.advisorKim, Tae-Kook-
dc.contributor.advisor김태국-
dc.contributor.authorChang, Ki-Young-
dc.contributor.author장기영-
dc.date.accessioned2011-12-12T08:54:22Z-
dc.date.available2011-12-12T08:54:22Z-
dc.date.issued2005-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243528&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/28054-
dc.description학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ iv, 42 p. ]-
dc.description.abstractDespite of the enormous knowledge accumulated about the genetic basis of human cancer, there have been only a few drugs which target the genetic differences between tumor cells and all normal cells in the body. To identify cancer-specific small molecules, a cell-based assay system which used co-culture strategy was newly developed. Co-culture of normal cells (WI38) and tumor cells (HCT-116) which expressed two different fluorescent proteins (YFP and CFP, respectively) allowed facile screening for cancer-specificity. Among 20,000 compounds screened, a novel compound, CGK3237, was identified that inhibited specifically the growth of HCT-116. Surprisingly, it was found out that only the growth of a certain type of cancer cells which had deregulated Wnt signaling pathway was selectively affected by CGK3237. By using a reporter assay for Wnt-dependent transcriptional activation, it was demonstrated that CGK3237 down-regulated the mRNA level of β -catenin and inhibited the occupancy of β-catenin onto TCF/LEF-responsive element. Consistent with this result, the activity of constitutively activated β-catenin in SW480 cells was also disturbed by CGK3237. The exact cellular target of CGK3237 was not identified yet, but these results suggest that chemical genomic approach is a very powerful method in searching for a novel anti-cancer drug.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectSialyltransferase-
dc.subjectGalactosyltransferase-
dc.subjectWnt/β-catenin pathwayosylation-
dc.subjectChemical Genomics-
dc.subjectDEAE Chromatographyv 2-D HPLCnjugation-
dc.subjectDEAE 크로마토그래피-
dc.subject시알산 전이효소-
dc.subject갈락토오스 전이효소-
dc.subjectWnt/β-catenin 경로-
dc.subject화학유전체학-
dc.titleChemical genomic studies of small molecule modulator of Wnt/β-catenin pathway = Wnt/β -catenin pathway의 저분자 화합물 조절인자에 관한 화학유전체학적 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN243528/325007 -
dc.description.department한국과학기술원 : 생명과학과, -
dc.identifier.uid020023524-
dc.contributor.localauthorKim, Tae-Kook-
dc.contributor.localauthor김태국-
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