DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Tae-Kook | - |
dc.contributor.advisor | 김태국 | - |
dc.contributor.author | Chang, Ki-Young | - |
dc.contributor.author | 장기영 | - |
dc.date.accessioned | 2011-12-12T08:54:22Z | - |
dc.date.available | 2011-12-12T08:54:22Z | - |
dc.date.issued | 2005 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243528&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28054 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ iv, 42 p. ] | - |
dc.description.abstract | Despite of the enormous knowledge accumulated about the genetic basis of human cancer, there have been only a few drugs which target the genetic differences between tumor cells and all normal cells in the body. To identify cancer-specific small molecules, a cell-based assay system which used co-culture strategy was newly developed. Co-culture of normal cells (WI38) and tumor cells (HCT-116) which expressed two different fluorescent proteins (YFP and CFP, respectively) allowed facile screening for cancer-specificity. Among 20,000 compounds screened, a novel compound, CGK3237, was identified that inhibited specifically the growth of HCT-116. Surprisingly, it was found out that only the growth of a certain type of cancer cells which had deregulated Wnt signaling pathway was selectively affected by CGK3237. By using a reporter assay for Wnt-dependent transcriptional activation, it was demonstrated that CGK3237 down-regulated the mRNA level of β -catenin and inhibited the occupancy of β-catenin onto TCF/LEF-responsive element. Consistent with this result, the activity of constitutively activated β-catenin in SW480 cells was also disturbed by CGK3237. The exact cellular target of CGK3237 was not identified yet, but these results suggest that chemical genomic approach is a very powerful method in searching for a novel anti-cancer drug. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Sialyltransferase | - |
dc.subject | Galactosyltransferase | - |
dc.subject | Wnt/β-catenin pathwayosylation | - |
dc.subject | Chemical Genomics | - |
dc.subject | DEAE Chromatographyv 2-D HPLCnjugation | - |
dc.subject | DEAE 크로마토그래피 | - |
dc.subject | 시알산 전이효소 | - |
dc.subject | 갈락토오스 전이효소 | - |
dc.subject | Wnt/β-catenin 경로 | - |
dc.subject | 화학유전체학 | - |
dc.title | Chemical genomic studies of small molecule modulator of Wnt/β-catenin pathway | - |
dc.title.alternative | Wnt/β -catenin pathway의 저분자 화합물 조절인자에 관한 화학유전체학적 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 243528/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020023524 | - |
dc.contributor.localauthor | Kim, Tae-Kook | - |
dc.contributor.localauthor | 김태국 | - |
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