Chemical genetic approach for screening insulin sensitizer = 인슐린 센서타이저의 개발

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Insulin signaling pathway is an evolutionary conserved mechanism. In organisms ranging from yeast to mouse, mutations in insulin or insulin-like receptors extend life span but also cause glycogen or fat accumulation and dwarfism. To develop insulin sensitizer, a simple diabetic model is adapted. DAF-2 is the only member of the insulin receptor family in whole C.elegans genome and if there is a mutation, worms are arrested on dauer larval stage instead of growing to adulthood. A chemical genetics approach is adopted to identify a novel potential drug for diabetes. If a molecule activates the down stream protein of insulin signaling pathway, the molecule can make daf-2 mutant worms overcome dauer and also, would be a insulin sensitizer. Three compounds that rescued dauer arrest were primarily screened out of a collection of 1120 compounds of a TGBz library and also, those compounds have the activity of increasing glucose uptake in 3T3 adiphocytes. Further studies show that the molecular target of the compounds is GAPDH, Glyceraldehyde 3-phospate dehydrogenase, and they act as a insulin sensitizer by inhibiting GAPDH activity.
Advisors
Byung, Si-Myung변시명
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
243514/325007  / 020023124
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2005.2, [ v, 44 p. ]

Keywords

step-in mode of AFM; insulin sensitizerourier transformv approximation; C.elegas; DAF-2; frequency analysis; 주파수 분석; 스텝 인 모드; 근사; 당뇨병산 푸리에 변환; 인슐린

URI
http://hdl.handle.net/10203/28042
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243514&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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