Genome-scale genetic screen of drosophila mutants showing abnormal temperature-preference behavior = 비정상적 온도 선호를 보이는 돌연변이 초파리의 게놈 수준에서의 유전학적 검색

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The perception of temperature in external environmental stimuli is one of the important sensory systems in organisms to survive. However, regarding the mechanisms of thermosensation little is known about it. Recently, a study of the mechanisms of thermosensation has been advanced by discovery of transient receptor potential (TRP) channels. These TRP channels are quite unusual in the sense that they react to their own temperature. Although, there are some TRP channels that respond to various temperatures - noxious heat, warm and cold temperature - in mammals, only two TRP channels are known in Drosophila that react to hot and warm temperature. Using Drosophila which has central nerve system as a model system, fourteen thousands independent lines of P-element inserted mutant flies were tested by temperature-preference behavior in genome-scale and then one hundred sixty two candidates were selected as thermomutants. Possibly affected genes in these lines were predated through database search. These hermomutants fall into several categories which are involved in regulation of transcription and translation, cytoskeleton, nervous system, cell communication, signal transduction, ubiquitination, metabolism, biosynthesis, G protein coupled receptor (GPCR), channel activity, other function and novel function. Using RT-PCR, the expression level of suspected genes was observed in central nerve system, epidermis, and guts of third instar larvae. Based on their domain homology, biological process, and expression level of each organ, sixteen thermomutants were selected. The expression patterns in central nerve system for these mutants were investigated by using in-situ hybridization method. Although, this high-throughput screen of genes is related to thermosensation, more detail research following this study will be clear up the mechanism of thermosensation form Drosophila to human.
Advisors
Kim, Jae-Seobresearcher김재섭researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
240338/325007  / 020033019
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2004.8, [ v, 55 p. ]

Keywords

DROSOPHILA TEMPERATURE SCREEN; 초파리 게놈 온도 선호

URI
http://hdl.handle.net/10203/28033
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=240338&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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