Characterization of drosophila parkin in vivo = 초파리 파킨 유전자의 기능 연구

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Parkin, encoding E3 ubiquitin-ligase is linked to Autosomal Recessive Juvenile Parkinsonism (AR-JP). The locomotive defects in Parkinson’s disease patients are known to be due to the selective loss of dopaminergic (DA) neurons. Here, I generated P-element inserted Dparkin null flies with abnormal wing posture and lower climbing ability. Intriguingly, I observed apoptotic phenotypes in Dparkin mutant indirect flight muscle (IFM), including mitochondrial swelling and TUNEL- positive nuclei. Dparkin mutant also showed elaborate branching of motor neurons on IFM. Using GAL4 system, I showed that Dparkin deficiency in DA neurons was responsible for IFM apoptosis. Meanwhile, elaborate neuronal branching, which was alleviated by pan-neuronal expression of exogenous Parkin, failed to be restored by either DA neuronal or muscle expression of exogenous Parkin. Moreover, Dparkin mutant showed reduced pre-synaptic vesicle protein (CSP)-positive signals on IFM, suggesting that Dparkin has a neuronal growth regulatory role at the neuro muscular junction (NMJ) by affecting synaptic transmission. Collectively, I demonstrate that Dparkin has essential roles in both muscle cell survival and motor neuronal branching control, strongly supporting the importance of Parkin in neurodegenerative movement disorder of Parkinson’s disease.
Advisors
Chung, Jong-Kyeongresearcher정종경researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
237819/325007  / 020023461
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2004.2, [ iv, 46 p ]

Keywords

PARKIN; DROSOPHILA; 초파리; 파킨

URI
http://hdl.handle.net/10203/28026
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237819&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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