Development of serum-free media for the production of recombinant proteins from rCHO cells using statistical design of hydrolysates mixture = 배지 첨가물의 혼합비율 최적화를 이용한 외부 단백질을 생산하는 재조합 CHO 세포주를 위한 무혈청 배지 개발

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To achieve enhanced cell growth and increased product concentration in serum free media, three non-animal derived hydrolysates were used as additive to serum free basal medium (SFM-G; SFM-YH2) developed by information of the previous work in our laboratory. According to the simplex lattice design, 10 media that have different proportions of yeastolate, soy hydrolysate, and wheat gluten hydrolysate were prepared. After Batch cultures of 3 rCHO cell lines producing anti-4-lbb antibody (H-1-5), hTPO (R-2-3-2), and ABC antibody (ABC 11-08) in 10 media prepared before, the effect of each medium was analyzed by statistical program. For H-1-5 and ABC 11-08 cells, yestolate showed strong effect on enhancing specific production rate (q) and negative effect on growth of cells. Wheat gluten hydrolysate showed strong effect on growth of cells when mixed with other hydrolysate. Soy hydrolysate showed positive (not highest) effect on growth and production. For R-2-3-2 cells, yeastolate was most suitable additives that do not need to mix with other hydrolysate. Then, batch cultures of 3 rCHO cell line using optimized proportions of 3 hydrolysates obtained by program were carried out to confirm the result predicted by the simulation. Further, for ABC 11-08 the effect of hydrolysates mixture on the growth of cells was maintained through over 6 passeges in SFM.
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
237806/325007  / 020023093
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2004.2, [ vi, 41 p. ]

Keywords

HYDROLYSATES MIXTURE; SERUM-FREE MEDIA; STATISTICAL DESIGN; 혼합비율 최적화; 배치 첨가물; 무혈청 배지

URI
http://hdl.handle.net/10203/28013
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237806&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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