Gene cloning, expression, and purification of mitochondrial transcription factor TFB2M in escherichia coli = 인체 미토콘드리아 전사 인자 TFB2M 클로닝, 발현과 정제

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Characterization of the basic transcription machinery of human mitochondrial DNA is of fundamental biological interest and may also lead to therapeutic interventions for human diseases associated with mitochondrial dysfunction. Recently it was reported that two forms of a transcription factor (TFB1M, TFB2M) have been identified as the missing components of the transcription initiation machinery for human mitochondrial DNA. We cloned the genes encoding TFB1M and TFB2M from a human cDNA library by polymerase chain reactions (PCR) and subcloned them into an expression vector. The genes were expressed in Escherichia coli (E.coli) and the proteins were purified by affinity chromatography. These, together with the transcription factor A (TFAM) and mitochondrial RNA polymerase (POLRMT), constitute the complete transcription initiation machinery in human mitochondria. The reconstituted system will be used to study the control of mitochondrial transcription and DNA replication.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
237804/325007  / 020023030
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2004.2, [ vi, 49 p. ]

Keywords

TRANSCRIPTION FACTOR; MITOCHONDRIA; TFB2M; TFB2M; 미토콘드리아 전사인자; 미토콘드리아 전사; 미토콘드리아

URI
http://hdl.handle.net/10203/28011
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237804&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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