Transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene in response to TGF-b treatment = TGF-β에 의한 hTERT 유전자의 전사조절에 대한 연구

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Telomerase is important in cellular proliferative activity. Human telomerase reverse transcription (hTERT) is the catalytic subunit and key component of telomerase. The transcription of hTERT is limit-rate step of telomerase. Telomerase activity decreases in response to TGF-β. Through transient reporter assay, we find that hTERT promoter activity decreases, too. To identify the major regulatory element which contributes this decline of promoter activity, serial deletion analysis was done. As the result, we find that TGF-β represses hTERT promoter via E2F-site. The result of reporter assay with single mutant constructs on hTERT promoter agrees with this fact. Many promoter repressed in G0/G1 arrest are regulated by E2F-pocket protein complex via E2F-site. Through Chromatin IP assay, we confirmed that E2F4 and p130 bind to hTERT promoter in TGF-β treated HaCaT cells. Therefore, we suppose that E2F4-p130 complex through E2F-binding site regulate the hTERT promoter activity in response to TGF-β.
Advisors
Chung, Jong-KyeongresearcherKim, Tae-Kookresearcher정종경researcher김태국researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2003
Identifier
230632/325007  / 020013398
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2003.8, [ iv, 47 p. ]

Keywords

TGF-beta; transcriptional regulation; E2F-pocket protein complex; 텔로머레이즈; 전사기작; 암화과정; Telomerase; hTERT

URI
http://hdl.handle.net/10203/28007
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=230632&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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