DC Field | Value | Language |
---|---|---|
dc.contributor.author | Youk, Jeonghwan | ko |
dc.contributor.author | Kim, Taewoo | ko |
dc.contributor.author | Evans, Kelly, V | ko |
dc.contributor.author | Jeong, Young-Il | ko |
dc.contributor.author | Hur, Yongsuk | ko |
dc.contributor.author | Hong, Seon Pyo | ko |
dc.contributor.author | Kim, Je Hyoung | ko |
dc.contributor.author | Yi, Kijong | ko |
dc.contributor.author | Kim, Su Yeon | ko |
dc.contributor.author | Na, Kwon Joong | ko |
dc.contributor.author | Bleazard, Thomas | ko |
dc.contributor.author | Kim, Ho Min | ko |
dc.contributor.author | Fellows, Mick | ko |
dc.contributor.author | Mahbubani, Krishnaa T. | ko |
dc.contributor.author | Saeb-Parsy, Kourosh | ko |
dc.contributor.author | Kim, Seon Young | ko |
dc.contributor.author | Kim, Young Tae | ko |
dc.contributor.author | Koh, Gou Young | ko |
dc.contributor.author | Choi, Byeong-Sun | ko |
dc.contributor.author | Ju, Young Seok | ko |
dc.contributor.author | Lee, Joo-Hyeon | ko |
dc.date.accessioned | 2021-01-28T05:53:12Z | - |
dc.date.available | 2021-01-28T05:53:12Z | - |
dc.date.created | 2021-01-19 | - |
dc.date.created | 2021-01-19 | - |
dc.date.created | 2021-01-19 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | CELL STEM CELL, v.27, no.6, pp.905 - + | - |
dc.identifier.issn | 1934-5909 | - |
dc.identifier.uri | http://hdl.handle.net/10203/280037 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of a present pandemic, infects human lung alveolar type 2 (hAT2) cells. Characterizing pathogenesis is crucial for developing vaccines and therapeutics. However. the lack of models mirroring the cellular physiology and pathology of hAT2 cells limits the study. Here, we develop a feeder-free, long-term, three-dimensional (3D) culture technique for hAT2 cells derived from primary human lung tissue and investigate infection response to SARS-CoV-2. By imaging-based analysis and single-cell transcriptome profiling, we reveal rapid viral replication and the increased expression of interferon-associated genes and proinflammatory genes in infected hAT2 cells, indicating a robust endogenous innate immune response. Further tracing of viral mutations acquired during transmission identifies full infection of individual cells effectively from a single viral entry. Our study provides deep insights into the pathogenesis of SARS-CoV-2 and the application of defined 3D hAT2 cultures as models for respiratory diseases. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2 | - |
dc.type | Article | - |
dc.identifier.wosid | 000596086200007 | - |
dc.identifier.scopusid | 2-s2.0-85095831044 | - |
dc.type.rims | ART | - |
dc.citation.volume | 27 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 905 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | CELL STEM CELL | - |
dc.identifier.doi | 10.1016/j.stem.2020.10.004 | - |
dc.contributor.localauthor | Kim, Ho Min | - |
dc.contributor.localauthor | Ju, Young Seok | - |
dc.contributor.nonIdAuthor | Evans, Kelly, V | - |
dc.contributor.nonIdAuthor | Jeong, Young-Il | - |
dc.contributor.nonIdAuthor | Hur, Yongsuk | - |
dc.contributor.nonIdAuthor | Hong, Seon Pyo | - |
dc.contributor.nonIdAuthor | Kim, Je Hyoung | - |
dc.contributor.nonIdAuthor | Na, Kwon Joong | - |
dc.contributor.nonIdAuthor | Bleazard, Thomas | - |
dc.contributor.nonIdAuthor | Fellows, Mick | - |
dc.contributor.nonIdAuthor | Mahbubani, Krishnaa T. | - |
dc.contributor.nonIdAuthor | Saeb-Parsy, Kourosh | - |
dc.contributor.nonIdAuthor | Kim, Seon Young | - |
dc.contributor.nonIdAuthor | Kim, Young Tae | - |
dc.contributor.nonIdAuthor | Koh, Gou Young | - |
dc.contributor.nonIdAuthor | Choi, Byeong-Sun | - |
dc.contributor.nonIdAuthor | Lee, Joo-Hyeon | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
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