(An) application of E. coli ornithine deacetylase (argE) gene as a negative selectable marker in plant = 대장균 ornithine deacetylase (argE) 유전자를 이용한 식물 선발 표지 유전자의 개발

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 385
  • Download : 0
Deacetylation of an inactive precursor of herbicide, N-acetyl phosphinothricin (N-AcPt) results in the active herbicide molecule, phosphinothricin (Pt). This process was applied on plant as a negative selection system. I isolated E. coli ornithine deacetylase (argE) gene and expressed this gene in tobacco under the control of constitutive Cauliflower mosaic virus (CaMV) 35S promoter. The herbicide, Pt was acetylated and purified by evaporation of acetic acid. And I established successful green-house level negative selection system by using N-AcPt, which was harmless to nontransgenic tobacco and only destroyed argE transgenic tobacco transformant selectively.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2003
Identifier
180018/325007 / 020013453
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2003.2, [ v, 40 p. ]

Keywords

plant; negative selectable marker; ornithine deacetylase; 식물; 선발 표지 유전자; phosphinothricin; argE

URI
http://hdl.handle.net/10203/28003
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=180018&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0