Characterization and application of new dendrimer-based platforms for the protein chip덴드리머 고분자를 이용한 새로운 단백질 칩 표면구조의 분석 및 응용 연구

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dc.contributor.advisorKim, Hak-Sung-
dc.contributor.advisor김학성-
dc.contributor.authorKim, Young-Ja-
dc.contributor.author김영자-
dc.date.accessioned2011-12-12T08:53:20Z-
dc.date.available2011-12-12T08:53:20Z-
dc.date.issued2003-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=180001&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/27986-
dc.description학위논문(석사) - 한국과학기술원 : 생물과학과, 2003.2, [ vi, 59 p. ]-
dc.description.abstractCHAPTER 1. Controlling Nonspecific Protein Binding in PEG(poly-ethylene glycol)-conjugated PAMAM Dendrimer Monolayers on Gold. PEG has been widely used as a coating material for biosensor applications because of biocompatibility, enhancing hydrophilicity and flexibility, and reducing non-specific binding (NSB) of proteins on a surface. This chapter describes characteristics of a PEG-conjugated PAMAM dendrimer and its monolayers as a new chip platform. Synthesis of PEG-conjugated fourth generation PAMAM dendrimer and its monolayer formation were identified by performing NMR analysis, Fourier transform infrared (FT-IR) spectroscopy, contact angle measurement and ellipsometry. And, nonspecific protein adsorption on PEG-conjugated PAMAM dendrimer monolayers was examined by conducting surface plasmon resonance (SPR) spectroscopy. According to an elemental analysis by 2D-NMR, $^1H-$, and $^{13}C-NMR$ analysis, the fourth generation PAMAM dendrimer (G4) as a starting material was PEGylated with a degree of 95 ± 2 %. From FT-IR spectroscopy on the monolayers of PEG-conjugated PAMAM dendrimer assembled on a MUA-SAM/Au surface, amide coupling between dendrimers and PEG groups and existence of a C-O-C ether linkage in a tethered PEG moiety were demonstrated. The surface wettability measurement on the monolayers of PEG-conjugated PAMAM dendrimers was 25.2 ± 2˚ of contact angle, more hydrophilic than PAMAM dendrimer monolayers of 31 ± 2˚. By ellipsometry, the film thickness of PEG-conjugated PAMAM dendrimer monolayer assembled on SAM/Au surface was 58 ± 2Å, suggesting substantial distortion of the layer on the flat Au surface. The NSB effects for typical proteins were investigated such as bovine serum albumin (BSA) and anti-bovine IgG. The PEG-containing surface was more resistant to non-specific protein adsorption, yielding nearly 10% NSB level for both proteins compared to those from PAMAM dendrimer monolayers. These results support that PEG-conjugated PAMAM dendrimer is ...eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectdendrimer-
dc.subjectprotein chip-
dc.subjectpolymer-
dc.subject표면분석-
dc.subject고분자-
dc.subject덴드리머-
dc.subject단백질 칩-
dc.titleCharacterization and application of new dendrimer-based platforms for the protein chip-
dc.title.alternative덴드리머 고분자를 이용한 새로운 단백질 칩 표면구조의 분석 및 응용 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN180001/325007-
dc.description.department한국과학기술원 : 생물과학과, -
dc.identifier.uid020013107-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.localauthor김학성-
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BS-Theses_Master(석사논문)
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