DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Hak-Sung | - |
dc.contributor.advisor | 김학성 | - |
dc.contributor.author | Kim, Young-Ja | - |
dc.contributor.author | 김영자 | - |
dc.date.accessioned | 2011-12-12T08:53:20Z | - |
dc.date.available | 2011-12-12T08:53:20Z | - |
dc.date.issued | 2003 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=180001&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27986 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2003.2, [ vi, 59 p. ] | - |
dc.description.abstract | CHAPTER 1. Controlling Nonspecific Protein Binding in PEG(poly-ethylene glycol)-conjugated PAMAM Dendrimer Monolayers on Gold. PEG has been widely used as a coating material for biosensor applications because of biocompatibility, enhancing hydrophilicity and flexibility, and reducing non-specific binding (NSB) of proteins on a surface. This chapter describes characteristics of a PEG-conjugated PAMAM dendrimer and its monolayers as a new chip platform. Synthesis of PEG-conjugated fourth generation PAMAM dendrimer and its monolayer formation were identified by performing NMR analysis, Fourier transform infrared (FT-IR) spectroscopy, contact angle measurement and ellipsometry. And, nonspecific protein adsorption on PEG-conjugated PAMAM dendrimer monolayers was examined by conducting surface plasmon resonance (SPR) spectroscopy. According to an elemental analysis by 2D-NMR, $^1H-$, and $^{13}C-NMR$ analysis, the fourth generation PAMAM dendrimer (G4) as a starting material was PEGylated with a degree of 95 ± 2 %. From FT-IR spectroscopy on the monolayers of PEG-conjugated PAMAM dendrimer assembled on a MUA-SAM/Au surface, amide coupling between dendrimers and PEG groups and existence of a C-O-C ether linkage in a tethered PEG moiety were demonstrated. The surface wettability measurement on the monolayers of PEG-conjugated PAMAM dendrimers was 25.2 ± 2˚ of contact angle, more hydrophilic than PAMAM dendrimer monolayers of 31 ± 2˚. By ellipsometry, the film thickness of PEG-conjugated PAMAM dendrimer monolayer assembled on SAM/Au surface was 58 ± 2Å, suggesting substantial distortion of the layer on the flat Au surface. The NSB effects for typical proteins were investigated such as bovine serum albumin (BSA) and anti-bovine IgG. The PEG-containing surface was more resistant to non-specific protein adsorption, yielding nearly 10% NSB level for both proteins compared to those from PAMAM dendrimer monolayers. These results support that PEG-conjugated PAMAM dendrimer is ... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | dendrimer | - |
dc.subject | protein chip | - |
dc.subject | polymer | - |
dc.subject | 표면분석 | - |
dc.subject | 고분자 | - |
dc.subject | 덴드리머 | - |
dc.subject | 단백질 칩 | - |
dc.title | Characterization and application of new dendrimer-based platforms for the protein chip | - |
dc.title.alternative | 덴드리머 고분자를 이용한 새로운 단백질 칩 표면구조의 분석 및 응용 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 180001/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 020013107 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.localauthor | 김학성 | - |
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