Biphasic culture strategy based on hyperosmotic pressure for improved humanized antibody production in Chinese hamster ovary cell culture = 재조합 CHO 세포에서 인간화항체의 생산 증대를 위한 고삼투압에 기반한 2단계 세포 배양 전략

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Hyperosmotic pressure increased specific antibody productivity ($q_Ab$) of recombinant CHO (rCHO) cells (SH2-0.32) while it depressed cell growth. Thus, the use of hyperosmolar medium did not increase the maximum antibody concentration substantially. To overcome this drawback, the feasibility of biphasic culture strategy was investigated. In the biphasic culture, cells were first cultivated in the standard medium with physiological osmolality (294 mOsm/kg) for cell growth. When cells reached the late exponential growth phase, the spent standard medium was replaced with the fresh hyperosmolar medium (522 mOsm/kg) for antibody production. The $q_Ab$ in growth phase with the standard medium was $2.1μg/10^{6}$ cells/day while the $q_Ab$ in antibody production phase with the hyperosmolar medium (522 mOsm/kg) was $11.1μg/$10^{6}$ cells/day. Northern blot analysis showed a positive relationship between the relative contents of intracellular immunoglobulin mRNA and $q_Ab$. Due to the enhanced $q_Ab$ and the increased cell concentration in biphasic culture, the maximum antibody concentration obtained in biphasic culture with 522 mOsm/kg medium exchange was 161% higher than that obtained in batch culture with the standard medium. Taken together, the simple biphasic culture strategy based on hyperosmotic culture is effective in improving antibody production of rCHO cells.
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2003
Identifier
180000/325007 / 020013077
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2003.2, [ v, 31 p. ]

Keywords

Chinese hamster ovary cells; biphasic culture; humanized antibody; 고삼투압; 인간화 항체; 2단계 세포 배양; hyperosmotic pressure

URI
http://hdl.handle.net/10203/27985
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=180000&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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