DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Byun, Si-Myung | - |
dc.contributor.advisor | 변시명 | - |
dc.contributor.author | So, Young-Sun | - |
dc.contributor.author | 소영선 | - |
dc.date.accessioned | 2011-12-12T08:53:13Z | - |
dc.date.available | 2011-12-12T08:53:13Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=177026&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27978 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.8, [ iv, 47 p. ] | - |
dc.description.abstract | A bacterial plasminogen activator, streptokinase is widely used as a thrombolytic agent in the treatment of myocardial infarction. In this study, streptokinase was modified by directed evolution. Through six rounds of random mutagenesis and three rounds of error-prone PCR, over 50 thousands of clones were screened by skim milk-plasminogen overlay test and plasminogen activation assay in 96-well plates. As a result, seven mutants with amino acid substitution were selected. Among them, S221F was purified and its amidolytic activity and plasminogen activation activity were analyzed at pH 7.4 and 37℃ with a chromogenic substrate, $NH_{2-D}-Val-Leu-Lys-p-nitroanilide. The S221F showed the 7.7-fold increased specific activity than wild-type SK owing to the decreased Michaelis constant for the substrate plasminogen. The evolved F221 may contribute to a hydrophobic core of SK β domain for substrate plasminogen binding. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | plasminogen | - |
dc.subject | streptokinase | - |
dc.subject | plasminogen activation | - |
dc.subject | 방향성 있는 인위적 분자 진화 | - |
dc.subject | 플라스미노젠 | - |
dc.subject | 스트렙토키나제 | - |
dc.subject | directed evolution | - |
dc.title | Enhancement of streptokinase activity by directed evolution | - |
dc.title.alternative | 인위적 분자 진화에 의한 스트렙토키나제 활성 증진 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 177026/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 020003247 | - |
dc.contributor.localauthor | Byun, Si-Myung | - |
dc.contributor.localauthor | 변시명 | - |
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