Restoration of defective GFP by insertion of random nucleotides무작위 뉴클레오타이드를 이용한 결함녹색형광단백질의 복원

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dc.contributor.advisorKim, Hak-Sung-
dc.contributor.advisor김학성-
dc.contributor.authorLee, Jeong-No-
dc.contributor.author이정노-
dc.date.accessioned2011-12-12T08:53:00Z-
dc.date.available2011-12-12T08:53:00Z-
dc.date.issued2002-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=173566&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/27964-
dc.description학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.2, [ iii, 41 p. ]-
dc.description.abstractThe study and understanding of the natural evolution of protein function clearly has important implications for the design of in vitro evolution strategies. Nature must explore enormously large sequence spaces in a manner that preferentially examines areas relatively high in function. There are many methods to create new function such as point mutation, error-prone PCR and DNA shuffling. Functional salvage screen(FSS) introduces randomly fragmented genomic DNA to the target protein. Therefore, FSS creates functionally new proteins that have a variety of sequence spaces. In order to control sequence spaces, we used specific size of random nucleotides. GFP was screened as a model protein for the directed evolution with random nucleotides. Defective GFP was constructed at the two positions. N-terminal one thirds(82Asp~89Pro) and two thirds(136Ile-145Tyr) loop regions of GFP(238aa) were firstly selected. And restoration of defective GFP with random nucleotides was executed with overlapping PCR technique. We screened 8 colonies that returned to original function at the two thirds loop region of GFP.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectgreen fluorescent protein-
dc.subjectdefective-
dc.subjectrestoration-
dc.subjectGFP-
dc.subject복원-
dc.subject결함녹색형광단백질-
dc.subject뉴클레오타이드-
dc.subject무작위-
dc.titleRestoration of defective GFP by insertion of random nucleotides-
dc.title.alternative무작위 뉴클레오타이드를 이용한 결함녹색형광단백질의 복원-
dc.typeThesis(Master)-
dc.identifier.CNRN173566/325007-
dc.description.department한국과학기술원 : 생물과학과, -
dc.identifier.uid020003418-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.localauthor김학성-
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