DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Hak-Sung | - |
dc.contributor.advisor | 김학성 | - |
dc.contributor.author | Kim, Tae-Hyoung | - |
dc.contributor.author | 김태형 | - |
dc.date.accessioned | 2011-12-12T08:52:50Z | - |
dc.date.available | 2011-12-12T08:52:50Z | - |
dc.date.issued | 2002 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=173555&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27953 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.2, [ v, 36 p. ] | - |
dc.description.abstract | Recombinant human epidermal growth factor (rhEGF) was conjugated with polyethylene glycol (PEG) to improve its physical stability during microencapsulation in biodegradable poly(lactic-co-glycolic acid) microspheres. rhEGF was conjugated with N-hydroxysuccimide (NHS)-derivatized methoxy-PEG (mPEG) of MW 2000 and 5000 under various reaction conditions to optimize the extent of pegylation. Pegylated rhEGF showed much enhanced physical stability against homogenization. Pegylated rhEGF was encapsulated in PLGA microspheres by a double emulsion solvent evaporation method to achieve a sustained release. Pegylated rhEGF exhibited a tri-phasic release profile with a reduced initial burst, compared to unpegylated rhEGF. This study demonstrated that protein pegylation enhanced physical stability of protein and could be a good approach to achieve a sustained protein release profile from biodegradable microspheres. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Microspheres | - |
dc.subject | PLGA | - |
dc.subject | PEGylation | - |
dc.subject | Recombinant human epidermal growth factor (rhEGF) | - |
dc.subject | Stability | - |
dc.subject | 안정성 | - |
dc.subject | 미립체 | - |
dc.subject | 폴리락틱글라이콜릭엑시드 | - |
dc.subject | 폴리글리이콜도입 | - |
dc.subject | 재조합 인간 표피세포 성장 인자 | - |
dc.title | Pegylated Recombinant Human Epidermal Growth Factor (rhEGF) for Sustained Release from Biodegradable PLGA Microspheres | - |
dc.title.alternative | 생분해성 PLGA 미립체로부터 폴리에틸렌 글리콜이 도입된 rhEGF의 지속적 방출에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 173555/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 020003144 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.localauthor | 김학성 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.