In vivo excision and amplification of the CFTR gene in the human BJAB cells = 인체 BJAB 세포내 CFTR 유전자의 분리와 증폭

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 361
  • Download : 0
In vivo excision and amplification of pre-determined, large genomic segments, directly from the genome of a natural host could be a novel cloning method for gene therapy. In this study, an in vivo excision and amplification system for human cells was devised with the Cre/loxP system of bacteriophage P1 and the large T antigen/SV40 on system of Simian virus 40. Two loxP sequences, each of which serves as a recognition site for recombinase Cre, were integrated unidirectionally into the 51- and 3``-end regions of the CFTR gene. SV40 on sequence, which serves as a conditional replication system, was inserted between the loxP sequences. Trans-acting gene cre and large T antigen, each of which was under the control of a tetracycline responsive promoter, were also inserted into the 5``- and 3``-end regions of the CFTR gene, respectively, by homologous recombination. The human BJAB cell line carrying the inserted elements was stably maintained until the excision and amplification functions were triggered by the induction of cre and large T antigen. Upon induction by doxycycline, the 250-kb CFTR genomic region of human chromosome 7 flanked by two loxP sites was excised. Our method is very useful for obtaining large genomic fragments in quantities directly from a natural host without using foreign hosts.
Advisors
Kim, Sun-Changresearcher김선창researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2002
Identifier
173554/325007 / 020003124
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2002.2, [ iii, 34 p. ]

Keywords

Excision; Large T antigen/SV40 ori system; Cre/loxP system; Amplification; 유전자 증폭; 유전자 절단; 낭포성 섬유증; CFTR gene

URI
http://hdl.handle.net/10203/27952
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=173554&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0