Isolation and characterization of a fruit-specific cDNA clone in ripening oriental melon (Cucumis melo. var kuwata) = 참외(Cucumis melo var. kuwata)의 성숙단계에서 과육 특이적으로 발현되는 유전자의 분리

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To investigate molecular changes during the ripening of oriental melon (Cucumis melo var. kuwata) and to isolate a fruit-specific, ripening up-regulated gene, differential screening was done. 8 cDNAs corresponding to mRNAs whose abundance were increased during ripening were isolated, among them were 1-Aminocyclopropane-1-carboxylate oxidase (ACC OXIDASE), Abscisic acid, stress, ripening regulated gene homologue (ASR gene), and polyubiquitin. The other cDNAs showed no significant homology with known protein sequence. The expression pattern and genomic structure of a cDNA clone which showed homology with ASR gene was characterized. Expression of isolated ASR homologue was fruit specific, and could be detectable during Immature Green stage, increased till Ripe stage, and slightly decreased during Over Ripe stage. Southern blot analysis revealed there are low copies of ASR gene homologue in oriental melon genome. Full cDNA sequence of ASR gene homologue was obtained by Rapid Amplification of cDNA End.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2001
Identifier
165541/325007 / 000993605
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2001.2, [ v, 37 p. ]

Keywords

oriental melon; fruit specific expression; ripening; differential screening; 참외; 과육 특이적인 유전자; 성숙 단계; northern blot

URI
http://hdl.handle.net/10203/27931
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165541&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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