Synthesis of lysophosphatidylethanolamine from lysophosphatidylcholine using phospholipase D from Streptomyces sp = Streptomyces sp. 유래의 포스포리파제 D를 이용한 라이소포스파티딜콜린으로부터 라이소포스파티딜에탄올아민의 합성

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Lysophosphatidylethanolamine (LPE) was synthesized from lysophosphatidylcholine (LPC) using phospholipase D (PLD) from Streptomyces KF932. The transphosphatidylation reaction was done in a buffer system. The product was analyzed by HPLC. The parameters examined were the effect of reaction temperature, time, pH of the buffer, $CaCl_2$ concentration and molar ratio of LPC to ethanolamine. The highest yield of LPE was obtained after 6 hr of reaction at 60℃, in a buffer of pH 6.0 and at molar ratio of LPC to ethanolamine of 1:20. Addition of $CaCl_2$ decreased the yield. The maximum yield of LPE was 12.3% (mol/mol).
Advisors
Rhee, Joon-Shick이준식
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2001
Identifier
165509/325007 / 000993383
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2001.2, [ iv, 24 p. ]

Keywords

lysophosphatidylethanolamine; lysophosphatidylcholine; 라이소포스포리피드; transphosphatidylation; phospholipase D

URI
http://hdl.handle.net/10203/27927
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165509&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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