Characterization of open reading frame 50 response elements in Kaposi's sarcoma-associated herpesvirus promoters = 카포시 육종 허피스 바이러스의 프로모터에 존재하는 open reading frame 50 단백질의 반응요소에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 268
  • Download : 0
Kaposi’s sarcoma-associated herpesvirus (KSHV) belongs to a human gammaherpesvirus that has a latent phase in host cells. ORF50 protein is the most important protein in reactivation to lytic phase and it is known as a strong transcriptional activator. Transactivation of promoters by ORF50 is done via response elements, so we tried to find ORF50 response elements in ORF50, K8, and ORF57 promoters with transient transfection assays of deletion mutants. We found smaller regions for activation by ORF50 in three promoters. The patterns of each response element are quite different and there is no conservative sequence, so ORF50 protein response elements are heterogeneous. AP-1 protein is participated in ORF57 promoter activation by ORF50, this result can be acquired by competitive electrophoretic mobility shift assay. ORF57 protein is a homolog of ICP27, a pleiotropic protein. We supposed that CBP is mediated because of the fact that ORF57 repress CREB mediated transcriptional pathway and p53 mediated transcriptional activation, in virto binding assay showed direct binding of CBP2 fragment (C/H3 domain) and C-terminus of ORF57. We can conclude ORF57 protein has strong repressor activity and CBP is related to this ORF57 activity
Advisors
Choe, Joon-Horesearcher최준호researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2001
Identifier
165497/325007 / 000993272
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2001.2, [ iii, 51 p. ]

Keywords

promoter; ORF50; KSHV; response elements; 반응요소; 프로모터; 바이러스; 육종; 카포시

URI
http://hdl.handle.net/10203/27926
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165497&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0