Cloning and characterization of the gene encoding bdellin from Hirudo nipponia = Hirudo nipponia 로부터 분리한 bdellin 유전자의 클로닝과 구조분석

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Bdellin is a potent trypsin, plamin and acrosin inhibitor purified from the leech. It is expressed in all parts of the body with high concentration in the reproductive organs.Bdellin-KL, bdellin from the Korean leech, has unsual hisitidine reach repeats which may be related to its function.But the physiological function in the leech has not revealed yet. To study on bdellin in gene level,the genomic clone encoding bdellin-KL was isolated from the constructed leech library.A positive clone containing the ORF which consists of 3 exons and 2 introns spanning about 1kb and 5``- and 3``- flanking region was isolated.The nucleotide sequences of exon-intron junctions were in agreement with the consensus 5``-GT …AG- 3``.To characterize the promoter, a transcription start point (tsp) was identified and the 5``-flanking sequence was analyzed.The consensus TATA box sequence (TATAAA) was found at - 28 bp from the tsp. Two CAAT boxes and two GC boxes were located upstream of the TATA box.Unusual ATA repeats were found upstream of the detected transcription factor binding sites.The promoter contained several putative cis-elements, which may serve as the binding sites of the important transcription factors related to the physiological function of bdellin.
Advisors
Kim, Sun-ChangresearcherKang, Ke-Wonresearcher김선창researcher강계원researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158716/325007 / 000983610
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ iv, 48 p. ]

Keywords

Gene cloning; Genomic library; 거머리; 델린; 유전자 클로닝; 유전자 도서관; Leech; Bdellin

URI
http://hdl.handle.net/10203/27916
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158716&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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