Cloning and sequence analysis of the gene encoding the guamerin from hirudo nipponia = 한국산 거머리에서 분리된 거머린의 유전자 클로닝과 염기 서열 분석

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Guamerin, a cysteine-rich elastase inhibitor of the Korean leech, Hirudo nipponia, is a specific inhibitor of elastase. A genomic DNA clone encoding guamerin was isolated from a λ BlueSTAR phage library constructed with Sau3AI partial digests of the chromosomal DNA of leeches, by screening with an guamerin cDNA as a probe. The gene of guamerin is about 1.2 kb long and composed of four exons and three introns. At the 5`` end of the coding region, there is a sequence that encodes a hydrophobic region of 19 amino acids, which could form a signal peptide. Primer extension analysis showed the transcription start point (tsp) was at A, 95 bp upstream from the start codon (ATG). The putative two CAAT boxes and the TATA box were found in the 5``-upstream region of the tsp. The 3``-flanking region contains three putative polyadenylation signals (AATAAA) 64 bp, 147 bp, and 232 bp downstream from the stop codon. From the pattern of the genomic Southern-blot analysis using a genomic DNA probe, the gene is considered to be at least two copy genes in the genome of the leech.
Advisors
Joe, Cheol-OresearcherKang, Ke-Wonresearcher조철오researcher강계원researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158714/325007 / 000983580
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ iv, 39 p. ]

Keywords

Genomic library; Genomic sequence; Transcription start point; 거머리; 일라스테이즈 억제제; 유전자 라이브러리; 유전자 서열; 전사 시작점; Leech; Elastase inhibitor

URI
http://hdl.handle.net/10203/27914
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158714&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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