Development of the screen-printed multi-biosensor for amperometric determination of LDH, ALT and AST levels in biological fluids = 스크린 프린팅 기술을 이용한 LDH, ALT 및 AST 측정용 후막형 전기화학식 멀티 바이오센서의 개발

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The screen-printed multi-biosensors for amperometric determination of LDH, ALT and AST levels in biological fluids have been constructed on the basis of $NAD^+$/NADH-dependent dehydrogenase reaction. The printing ink used for the fabrication of working electrode where enzyme-catalyzed reaction takes place is composed of reagents for enzyme reaction, polymer binder, 3,4-dihydroxybenzaldehyde (3,4-DHB) as electron-transfer mediator and graphite particle as conducting material. The printing-step is followed by electrodeposition of 3,4-DHB by potential cycling from -200 mV to +300 mV vs. standard Ag/AgCl reference electrode. Voltammetric and amperometric studies show that the multi-biosensor modified with 3,4-DHB could efficiently catalyze electrochemical oxidation of NADH generated by enzyme reaction, which indicate that electrode fouling due to direct electrochemical oxidation of NADH can be bypassed through this approach. Optimal conditions for the electrochemical determination of LDH, ALT and AST have been also investigated in terms of sensitivity. The calibration curve of the multi-biosensor for LDH level exhibits linear range up to 500 U/L and the detection limit is 50 U/L. In cases of the electrochemical determination of ALT and AST levels, both calibration curves of the multi- biosensor show linear range up to 600 U/L and the detection limits are 10 U/L in common.
Kim, Hak-Sungresearcher김학성researcher
한국과학기술원 : 생물과학과,
Issue Date
158711/325007 / 000983503

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ v, 54 p. ]


Alanine aminotransferase (ALT); Lactate dehydrogenase (LDH); Screen-printing technology; Amperometric biosensor; Aspartate aminotransferase (AST); 아스파테이트 아미노기 전이효소; 알라닌 아미노기 전이효소; 젖산 탈수소 효소; 스크린 프린팅 기술; 전기화학식 바이오센서

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