Transesterification of phosphatidylcholine with eicosapentaenoic acid using phospholipase $A_2$ in organic solvent = 포스포리파제 $A_2$를 이용한 eicosapentaenoic acid를 함유하는 포스타티딜콜린의 유기용매 내에서의 합성

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Phosphatidylcholine (PC) containing eicosapentaenoic acid (EPA C 20:5) in the sn - 2 position, was synthesized by phospholipase $A_2$ (porcine pancreatic phospholipase, $PLA_2$) mediated transesterification. The phospholipid modification was carried out in organic solvent media. The studied parameters were the effect of different solvents, time, water activity, acyl donor concentrations, the amount of $PLA_2$, and the pH of $PLA_2$. The modification was possible only when EPA ethyl ester (EPAEE) was used as an acyl donor in organic solvent system. The optimal conditions for the transesterification of the phosphatidylcholine were obtained by phosphatidylcholine and EPA ethyl ester (molar ratio of PC to EPAEE 1:10) in the presence of 10 mg (11 unit /mg) $PLA_2$ (pH 8.2) in 5 ml toluene at 50℃ for 48 h with the initial water activity ($a_w$) 0.25. The maximum incorporation of EPA into PC was 19.9% (w/w)
Advisors
Rhee, Joon-Shick이준식
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158698/325007 / 000983236
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ v, 36 p. ]

Keywords

포스포리파제; 다가불포화지방산; 효소적 합성; Transesterification; Phospholipase; PUFA; Enzymatic synthesis; 트랜스에스터리피케이션

URI
http://hdl.handle.net/10203/27898
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158698&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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