Microencapsulation of human growth hormone within biodegradable polyester microspheres : Protein aggregation stability and incomplete release mechanism = 인간성장호르몬의 생분해성 폴리에스터 미립구내로의 봉입 : 단백질의 응집 안정성과 불완전한 방출거동 기작에 관한 연구Protein aggregation stability and incomplete release mechanism

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 275
  • Download : 0
Recombinant human growth hormone (rhGH) was encapsulated within poly(D,L-lactic-co-glycolic acid) microspheres by a double emulsion solvent evaporation method. A mixture of methylene chloride and ethyl acetate in varying volume ratios was used for the microsphere preparation. Protein release profiles from three different microsphere formulations demonstrated initial burst effects ranging from 28.2 to 54.7 % after one day incubation, and exhibited no further significant releases up to 19 days. This was because the encapsulated rhGH with the microspheres was largely aggregated in a noncovalent fashion during the formulation. Non-aggregated water soluble rhGH species within the microspheres are likely to be responsible for the rapid release upon incubation. The initially released rhGH in the incubation medium, however, was composed of mostly monomer species with a small amount of dimer as probed by size exclusion chromatography. Circular dichroism spectra of the initially released rhGH in the medium revealed that the conformation of the released rhGH was correctly folded relative to that of native rhGH, with little variation in a-helix contents depending on the formulations. The "non-release" mechanism after the initial burst release was attributed to non-spontaneously dissociable non-covalent protein aggregation and surface adsorption of rhGH present within the microspheres.
Advisors
Park, Tae-Gwanresearcher박태관researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158696/325007 / 000983172
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ viii, 46 p. ]

Keywords

Microsphere; PLGA; Human growth hormone; Protein delivery; Biodegradable; 생분해성; 미립구; PLGA; 인간성장호르몬; 단백질 전달

URI
http://hdl.handle.net/10203/27896
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158696&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0