Regulation of STAT3 transcription activity by replication protein A 32 kDa = Replication Protein A 32KDa 에 의한 STAT3 전사인자의 활성조절

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STATs (signal transducers and activators of transcription) are transcription factors that contain SH2 domains and are activated by tyrosine phosphorylation in response to cytokines and growth factors. Replication protein A (RPA) is a heterotrimeric complex that consists of three subunits, p70, p32 and p11, and has important functions in DNA replication and metabolism. Here, I present evidence that the RPA p32 subunit specifically binds to the SH2 domain of STAT3 in a phosphotyrosine-independent manner. I confirm their protein-protein interactions by yeast 2-hybrid analyses and in vitro binding assays using recombinant proteins generated from bacteria and in vitro translation. I also show that STAT3 binds to RPA p32 in vivo by conducting co-precipitation experiments. As the SH2 domain is highly involved in the tyrosine phosphorylation and the transcriptional activity of STAT3, over-expression of RPA p32 correspondingly augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of STAT3.
Advisors
Chung, Jong-Kyeongresearcher정종경researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158693/325007 / 000983133
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ v, 41 p. ]

Keywords

STAT transcription factor; SH2 domain; Tyrosine phosphorylation; RPA p32; RPA p32; STAT 전사인자; SH2 도메인; 타이로신 인산화

URI
http://hdl.handle.net/10203/27893
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158693&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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