Screening of interferon inducers and its induction mechanism = 인테페론 유도물질의 선별과 유도 기작에 관한 연구

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Interferons are very effective species specific glycoprotein which has antiviral, immunomodulatory and antiproliferative properties. Fibroblast interferon (F interferon, 1FN-$\beta$) was produced from FS-4 cell line by superinduction method with treatment of synthetic poly I:poly C and various inducers. Produced interferon had apparent antiviral activity against the challenge of Indiana-type vesicular stomatitis virus (VSV). To select nontoxic and safe inducers, the extracts of natural products that were known as anticancer substances were tested interferon-induced capacity. But all of them were ineffective to induce interferon. For enhancement of uptake of poly I:poly C into the cells, $CaCl_2$ and DMSO were treated to cells. But the result was not encouraged. Denaturation of poly I:poly C had decreasing of inducing activity and priming effect of cells with small amount of interferon was also observed. When polyanion material, especially heparin, was treated to cells after removing of antimetabolites, the level of interferon was two or three times than that of normal superinduction. It was thought that the competition effect of heparin was in shutoff mechanism of interferon mRNA. Interferon and polyanion materials were treated to cells together. At this time, antiviral activity of interferon was slightly diminished because the polyanion substances are nuclease inhibitors.
Advisors
Byun, Si-Myung변시명
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1982
Identifier
63304/325007 / 000801023
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ 89 p. ]

URI
http://hdl.handle.net/10203/27875
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=63304&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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