This study proposes a scheme to identify insider threats in nuclear facilities through the detection of malicious intentions of potential insiders using subject-wise classification. Based on electroencephalography (EEG) signals, a classification model was developed to identify whether a subject has a malicious intention under scenarios of being forced to become an insider threat. The model also distinguishes insider threat scenarios from everyday conflict scenarios. To support model development, 21-channel EEG signals were measured on 25 healthy subjects, and sets of features were extracted from the time, time-frequency, frequency and nonlinear domains. To select the best use of the available features, automatic selection was performed by random-forest-based algorithms. The k-nearest neighbor, support vector machine with radial kernel, naive Bayes, and multilayer perceptron algorithms were applied for the classification. By using EEG signals obtained while contemplating becoming an insider threat, the subject-wise model identified malicious intentions with 78.57% accuracy. The model also distinguished insider threat scenarios from everyday conflict scenarios with 93.47% accuracy. These findings could be utilized to support the development of insider threat mitigation systems along with existing trustworthiness assessments in the nuclear industry.