DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Hyun-Jae | - |
dc.contributor.advisor | 이현재 | - |
dc.contributor.author | Choi, Eui-Seong | - |
dc.contributor.author | 최의성 | - |
dc.date.accessioned | 2011-12-12T08:51:12Z | - |
dc.date.available | 2011-12-12T08:51:12Z | - |
dc.date.issued | 1980 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=62644&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27846 | - |
dc.description | 학위논문 (석사) - 한국과학기술원 : 생물공학과, 1980, [ vi, 69 p. ] | - |
dc.description.abstract | The L-sorbose dehydrogenase which catalyzes the oxidation of L-sorbose to L-sorbosone was isolated from G. melanogenus ATCC 15163 by fractional sedimentation as a membrane-bound particulate form and its general properties were characterized. It was found that the enzyme consumes oxygen or reduces certain dyes such as PMS, MB, and $K_3Fe(CN)_6$ as electron acceptors for the specific oxidation of L-sorbose to L-sorbosone. The stoichiometry of the enzyme reaction was established; L-sorbose + 1/2 $O_2 → L-sorbosone + $H_2O$. The enzyme activity was assayed colorimetrically by measuring the production of L-sorbosone using MBTH and $FeCl_3$. This colorimetric method was very specific and sensitive. The enzyme was found to be extremely specific toward L-sorbose with a Km value of 7.7 mM and it showed slight binding affinities for D-glucose and D-xylose which have a D-xylo configuration. The maximum activity was obtained at pH 7.5 and 45 ℃. The enzyme activity was completely inhibited by heavy metal ions such as $Ag^{+2}$, $Hg^{+2}$, and $Mo^{+2}$. No requirement of coenzymes and free metal ions was found. It is suggested that the enzyme is linked to the cytochrome system in the respiratory chain. Also, a sequential kinetic mechanism was shown in the binding of two substrate, L-sorbose and oxygen, to the complex in which the anzyme is linked to the respiratory chain. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 단백질 분리. | - |
dc.title | Isolation and characterization of L-sorbose dehydrogenase from gluconobacter melanogenus ATCC 15163 | - |
dc.title.alternative | Sorbose dehydrogenase 의 추출 및 특성에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 62644/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000781208 | - |
dc.contributor.localauthor | Lee, Hyun-Jae | - |
dc.contributor.localauthor | 이현재 | - |
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