Study on the plasminogen activation by microbial activators : streptokinase and staphylokinase = Streptokinase 와 staphylokinase 에 의한 plasminogen 의 활성화에 대한 연구streptokinase and staphylokinase

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 310
  • Download : 0
The activation of plasminogen to an active fibrinolytic enzyme, plasmin, by Streptokinase and Staphylokinase was studied using caseinolytic assay method. The Staphylokinase producing microorganism was selected among 15 $\beta$-hemolytic Staphylococci and that of Streptokinase was purchased from ATCC. Staphylokinase was purified by CM-cellulose ion exchange chromatography and Seph 4B-EACA column chromatography. Ammonium sulfate fractionation and DEAE-cellulose ion exchange chromatography were used to purify Streptokinase. Both of them showed a very limitted substrate specificity toward plasminogen, and their catalytic activities were inhibited by the presence of macromolecular proteins such bovine serum albumin and casein. Both activators showed a similar mode of catalytic action on plasminogen, but their nature and molecular properties were found to be different from that of human urokinase. Based on kinetic experiments and other inhibition study, the reaction mode of these two activators was discussed. Also, by chemical modification, the ionic interaction between Streptokinase and Plasminogen to form complex was stuied.
Advisors
Lee, Hyun-Jae이현재
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1980
Identifier
62633/325007 / 000781075
Language
eng
Description

학위논문 (석사) - 한국과학기술원 : 생물공학과, 1980.2, [ [vii], 55 p. ]

URI
http://hdl.handle.net/10203/27835
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=62633&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0