(A) study on lipoxygenase from soybean = 대두의 lipoxygenase 에 관한 연구

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dc.contributor.advisorByun, Si-Myung-
dc.contributor.advisor변시명-
dc.contributor.authorKim, Kil-Hyoun-
dc.contributor.author김길현-
dc.date.accessioned2011-12-12T08:51:01Z-
dc.date.available2011-12-12T08:51:01Z-
dc.date.issued1980-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=62632&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/27834-
dc.description학위논문 (석사) - 한국과학기술원 : 생물공학과, 1980.2, [ viii, 67 p. ]-
dc.description.abstractSoybean lipoxygenase (E.C.1.13.11.12) was purified by ammonium sulfate fractionation, affinity chromatography on linoleate-aminohexyl Sepharose 4B and DEAE-cellulose chromatography. Purification of lipoxygenase-2 through affinity chromatography at PH 6.8 resulted in 6-fold purification and yield of 91%. However, linoleate-aminoethyl Sepharose 4B did not retain a significant amount of lipoxygenase. Further purification was conducted through DEAE-cellulose chromatography with the factor of 4-fold, and the yield was 46 % with respect to lipoxygenase-2. Overall purification of lipoxygenase-2 was 32.4-fold, and yield was 28.5 %. The purfied lipoxygenase-2 was almost homogeneous, electrophoretically. The Km values of lipoxygenase-1 and -2 for linoleic acid were 0.15 mM and 0.39 mM, respectively. And the activation energies of the reactions catalyzed by lipoxygenase-1 and -2 were 16.2 kcal/mole and 39.6 kcal/mole, respectively. Molecular weight of lipoxygenase was determined from gel chromatography on Sephadex G-150 to be 102,000. When lipoxygenase-2 was subjected to be reacted with arachidonic acid, and subsequently reduced with sodium dithionite, a material from ether extracts of this reaction mixture seemed to have some of prostaglandin F_{2α}, which was identified through thin layer chromatography.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subject단백질 분리.-
dc.title(A) study on lipoxygenase from soybean = 대두의 lipoxygenase 에 관한 연구-
dc.typeThesis(Master)-
dc.identifier.CNRN62632/325007-
dc.description.department한국과학기술원 : 생물공학과, -
dc.identifier.uid000781014-
dc.contributor.localauthorByun, Si-Myung-
dc.contributor.localauthor변시명-
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