DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Ryu, Deway D. Y. | - |
dc.contributor.advisor | 유두영 | - |
dc.contributor.author | Lee, Myo-Jae | - |
dc.contributor.author | 이묘재 | - |
dc.date.accessioned | 2011-12-12T08:50:40Z | - |
dc.date.available | 2011-12-12T08:50:40Z | - |
dc.date.issued | 1978 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=62260&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27809 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1978.2, [ vi, 57 p. ] | - |
dc.description.abstract | Using $\mbox{\underline{Micromonospora}}$ $\mbox{\underline{purourea}}$ strain, gentamicin was produced by fermentation. In this thesis, the increase of gentamicin productivity was studied by strain improvement \& systematic optimization of some important fermentation process variables. The gentamicin producing wild strain of $\mbox{\underline{M}}$. $\mbox{\underline{purpures}}$ (ATCC 15835) was improved to a high yielding strain by natural selection, irradiation with ultraviolet, induction of gentamicin resistance. Effective condition of U.V. irradiation was studied for repeated treatment. The resistance-induced mutant gave higher productivity than those mutant obtained by only/U.V. treatment. The growth characteristic of high yielding mutant differed from that of the wild strain; e.g. colony size, colony morphology, color of colony \& broth culture, growth rate etc. Slow germination of $\mbox{\underline{M}}$. $\mbox{\underline{purpurea}}$ changed to rapid rate by adjusting the pH of the germination broth to slightly alkaline condition. Potato starch \& extracted soy bean meal as the best carbon \& nitrogen sources for gentamicin fermentation were selected. The effect of metal ion such as $Mg^{++}$ ion \& $Co^{++}$ ion was tested. Addition of 10 mM -20 mM of $Mg^{++}$ ion to the fermentation medium didn``t effect positively on gentamicin production. Optimum concentration of $Co^{++}$ ion for gentamicin production was 0.08 g $CoCl_2$/ L broth. As the important fermentation process variables besides the medium, oxygen uptake rate and carbohydrate (Potato starch \& Glucose) uptake rate were measured. Maximum oxygen uptake rate was 6.25 mM $O_2$/L broth. HR. Maximum carbohydrate uptake rate was 0.8g glucose/L broth. HR and 0.41 g potato starch/L broth. HR. Also high sensitivity of the yield to the oxygen supply was observed. The optimum pH for the cell growth phase \& gentamicin production phase were different each other, they were 7.2 \& 6.8-6.9 respectively. The... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Studies on the production of gentamicin by fermentation | - |
dc.title.alternative | 발효에 의한 Gentamicin 생산에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 62260/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000761090 | - |
dc.contributor.localauthor | Ryu, Deway D. Y. | - |
dc.contributor.localauthor | 유두영 | - |
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