DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tian, Yuan | ko |
dc.contributor.author | Simanshu, Dhirendra K. | ko |
dc.contributor.author | Ma, Jin-Biao | ko |
dc.contributor.author | Park, Jong-Eun | ko |
dc.contributor.author | Heo, Inha | ko |
dc.contributor.author | Kim, V. Narry | ko |
dc.contributor.author | Patel, Dinshaw J. | ko |
dc.date.accessioned | 2020-12-04T01:10:15Z | - |
dc.date.available | 2020-12-04T01:10:15Z | - |
dc.date.created | 2020-12-04 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | MOLECULAR CELL, v.53, no.4, pp.606 - 616 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/278034 | - |
dc.description.abstract | We have solved two families of crystal structures of the human Dicer "platform-PAZ-connector helix'' cassette in complex with small interfering RNAs (siRNAs). The structures possess two adjacently positioned pockets: a 2 nt 3'-overhang-binding pocket within the PAZ domain (3' pocket) and a phosphate-binding pocket within the platform domain (phosphate pocket). One family of complexes contains a knob-like alpha-helical protrusion, designated "hDicer-specific helix,'' that separates the two pockets and orients the bound siRNA away from the surface of Dicer, which could be indicative of a product release/transfer state. In the second complex, the helical protrusion is melted/disordered and the bound siRNA is aligned toward the surface of Dicer, suggestive of a cleavage-competent state. These structures allow us to propose that the transition from the cleavage-competent to the postulated product release/transfer state may involve release of the 5'-phosphate from the phosphate pocket while retaining the 3' overhang in the 3' pocket. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer | - |
dc.type | Article | - |
dc.identifier.wosid | 000331660200009 | - |
dc.identifier.scopusid | 2-s2.0-84894274509 | - |
dc.type.rims | ART | - |
dc.citation.volume | 53 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 606 | - |
dc.citation.endingpage | 616 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2014.01.003 | - |
dc.contributor.localauthor | Park, Jong-Eun | - |
dc.contributor.nonIdAuthor | Tian, Yuan | - |
dc.contributor.nonIdAuthor | Simanshu, Dhirendra K. | - |
dc.contributor.nonIdAuthor | Ma, Jin-Biao | - |
dc.contributor.nonIdAuthor | Heo, Inha | - |
dc.contributor.nonIdAuthor | Kim, V. Narry | - |
dc.contributor.nonIdAuthor | Patel, Dinshaw J. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | CAENORHABDITIS-ELEGANS | - |
dc.subject.keywordPlus | MICROPROCESSOR COMPLEX | - |
dc.subject.keywordPlus | DROSHA-DGCR8 COMPLEX | - |
dc.subject.keywordPlus | PRIMARY MICRORNAS | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | RIBONUCLEASE | - |
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