Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes

Cited 67 time in webofscience Cited 35 time in scopus
  • Hit : 324
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorLavaert, Mariekeko
dc.contributor.authorLiang, Kai Lingko
dc.contributor.authorVandamme, Nielsko
dc.contributor.authorPark, Jong-Eunko
dc.contributor.authorRoels, Julietteko
dc.contributor.authorKowalczyk, Monica S.ko
dc.contributor.authorLi, Boko
dc.contributor.authorAshenberg, Orrko
dc.contributor.authorTabaka, Marcinko
dc.contributor.authorDionne, Danielleko
dc.contributor.authorTickle, Timothy L.ko
dc.contributor.authorSlyper, Michalko
dc.contributor.authorRozenblatt-Rosen, Oritko
dc.contributor.authorVandekerckhove, Bartko
dc.contributor.authorLeclercq, Georgesko
dc.contributor.authorRegev, Avivko
dc.contributor.authorVan Vlierberghe, Pieterko
dc.contributor.authorGuilliams, Martinko
dc.contributor.authorTeichmann, Sarah A.ko
dc.contributor.authorSaeys, Yvanko
dc.contributor.authorTaghon, Tomko
dc.date.accessioned2020-12-04T01:10:05Z-
dc.date.available2020-12-04T01:10:05Z-
dc.date.created2020-12-04-
dc.date.created2020-12-04-
dc.date.issued2020-06-
dc.identifier.citationIMMUNITY, v.52, no.6, pp.1088 - +-
dc.identifier.issn1074-7613-
dc.identifier.urihttp://hdl.handle.net/10203/278030-
dc.description.abstractDuring postnatal life, thymopoiesis depends on the continuous colonization of the thymus by bone-marrow-derived hematopoietic progenitors that migrate through the bloodstream. The current understanding of the nature of thymic immigrants is largely based on datafrom pre-clinical models. Here, we employed single-cell RNA sequencing (scRNA-seq) to examine the immature postnatal thymocyte population in humans. Integration of bone marrow and peripheral blood precursor datasets identified two putative thymus seeding progenitors that varied in expression of CD7; CD10; and the homing receptors CCR7, CCR9, and ITGB7. Whereas both precursors supported T cell development, only one contributed to intrathymic dendritic cell (DC) differentiation, predominantly of plasmacytoid dendritic cells. Trajectory inference delineated the transcriptional dynamics underlying early human T lineage development, enabling prediction of transcription factor (TF) modules that drive stage-specific steps of human T cell development. This comprehensive dataset defines the expression signature of immature human thymocytes and provides a resource for the further study of human thymopoiesis.-
dc.languageEnglish-
dc.publisherCELL PRESS-
dc.titleIntegrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes-
dc.typeArticle-
dc.identifier.wosid000541890000020-
dc.identifier.scopusid2-s2.0-85086606635-
dc.type.rimsART-
dc.citation.volume52-
dc.citation.issue6-
dc.citation.beginningpage1088-
dc.citation.endingpage+-
dc.citation.publicationnameIMMUNITY-
dc.identifier.doi10.1016/j.immuni.2020.03.019-
dc.contributor.localauthorPark, Jong-Eun-
dc.contributor.nonIdAuthorLavaert, Marieke-
dc.contributor.nonIdAuthorLiang, Kai Ling-
dc.contributor.nonIdAuthorVandamme, Niels-
dc.contributor.nonIdAuthorRoels, Juliette-
dc.contributor.nonIdAuthorKowalczyk, Monica S.-
dc.contributor.nonIdAuthorLi, Bo-
dc.contributor.nonIdAuthorAshenberg, Orr-
dc.contributor.nonIdAuthorTabaka, Marcin-
dc.contributor.nonIdAuthorDionne, Danielle-
dc.contributor.nonIdAuthorTickle, Timothy L.-
dc.contributor.nonIdAuthorSlyper, Michal-
dc.contributor.nonIdAuthorRozenblatt-Rosen, Orit-
dc.contributor.nonIdAuthorVandekerckhove, Bart-
dc.contributor.nonIdAuthorLeclercq, Georges-
dc.contributor.nonIdAuthorRegev, Aviv-
dc.contributor.nonIdAuthorVan Vlierberghe, Pieter-
dc.contributor.nonIdAuthorGuilliams, Martin-
dc.contributor.nonIdAuthorTeichmann, Sarah A.-
dc.contributor.nonIdAuthorSaeys, Yvan-
dc.contributor.nonIdAuthorTaghon, Tom-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusKILLER-CELL DEVELOPMENT-
dc.subject.keywordPlusT-CELL-
dc.subject.keywordPlusNATURAL-KILLER-
dc.subject.keywordPlusLINEAGE COMMITMENT-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusHEMATOPOIETIC PROGENITORS-
dc.subject.keywordPlusDENDRITIC CELLS-
dc.subject.keywordPlusB-CELLS-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusINNATE-
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 67 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0