Production of glucose isomerase from streptomyces species : optimization of medium and enzymatic properties of glucose isomerase = 방선균으로부터 포도당 이성화 효소의 생산에 관하여optimization of medium and enzymatic properties of glucose isomerase

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$\underline{Streptomyces}$ sp. HP produced glucose isomerase(EC 5.3.1. 5 D-xylose ketol-isomerase) and the enzyme yield was high when growth in the medium containing xylose or xylan as carbon source. Xylose was more effective inducer than xylan. By acid hydrolysis of wheat bran, about 70% of xylan in wheat bran was converted to xylose. By using hydrolyzed wheat bran, the optimal composition of medium was determined. After optimization of medium, the enzyme activity of microbial cells grown in hydrolyzed wheat bran medium was increased four times more than that of microbial cells grown in untreated wheat bran medium. Also some enzymatic properties of soluble enzyme were studied. The enzyme was very stable against heat and had an optimum pH at 8.0. Michaelis - Menten constants of forward and backward reactions were 0.196M and 0.392M respectively.
Advisors
Ryu, D.Y.유두영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1977
Identifier
62121/325007 / 000751125
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1977, [ [vi], 54 p. ]

URI
http://hdl.handle.net/10203/27796
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=62121&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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