Effects of minerals and carbon sources on chlortetracycline fermentation = 탄소공급원 및 무기염류가 클로로테트라 싸이클린 발효에 미치는 영향

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Medium improvement was studied because chlortetracycline was not produced during fermentation by the medium composition which were commonly shown in many previous works. We found many factors which affect for the production of chlortetracycline by the modification of the medium. Among them, the concentration of divalent ions and the kind of carbohydrate were rather important. The concentration of divalent ions above the concentration included in the natural medium, shows negative effect on the production of chlortetracycline. In many previous works, sucrose was used as main carbon source on chlortetracycline fermentation, but it was found that starch was rather good for chlortetracycline fermentation. A spectrophotometric assay method involving the extraction of chlortetracycline with butanol(1) under controlled conditions has been developed. By use of this procedure, the chlortetracycline content of both fermented and pharmaceutical products may be determined. This method is applicable to quality control. In addition, the determination of cell growth was carried out by measuring the total hyphal length. This method showed rather good result for the determination of cell growth of Streptomyces sp. which grows well in the complexed medium where many insoluble solid contents are contained.
Advisors
Ryu, Dewey유두영
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1976
Identifier
61959/325007 / 000741012
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1976.2, [ [ii], 46 p. ]

URI
http://hdl.handle.net/10203/27763
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=61959&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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