Structural and functional role of his 67 residue in subtilisin E = 서브틸리신 E 에서 His 67 잔기의 구조및 기능적 역할

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dc.contributor.advisorByun, Si-Myung-
dc.contributor.advisor변시명-
dc.contributor.authorLee, Si-Hyoung-
dc.contributor.author이시형-
dc.date.accessioned2011-12-12T08:49:49Z-
dc.date.available2011-12-12T08:49:49Z-
dc.date.issued1992-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60366&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/27750-
dc.description학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ vii, 42 p. ]-
dc.description.abstractHis 67 residue is highly conserved among subtilisin family and changed to Arg in mammalian subtilisin-like proteins. To elucdate the structural and functional role of His 67 residue in subtilisin E, site-directed mutagenesis was carried out. Three mutants of subtilisin E with Ala 67, Glu 67 and Gly 67 were obtained. Each mutant was characterized in terms of proteolytic activity. Compared with wild type, proteolytic activity for Ala 67 mutant was about 30\%. Glu 67 and Gly 67 mutant did not show any detectable proteolytic activity on Nsuccinyl- L- Ala- L- Ala- L- Pro- L- Phe- p- nitroanilide but developed hallow zones onto the skim-milk plate. It is expected that His 67 residue in subtilisin E influences on catalytic efficiency.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subject부위 특이 돌연변이-
dc.titleStructural and functional role of his 67 residue in subtilisin E = 서브틸리신 E 에서 His 67 잔기의 구조및 기능적 역할-
dc.typeThesis(Master)-
dc.identifier.CNRN60366/325007-
dc.description.department한국과학기술원 : 생물공학과, -
dc.identifier.uid000901376-
dc.contributor.localauthorByun, Si-Myung-
dc.contributor.localauthor변시명-
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BS-Theses_Master(석사논문)
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