Effects of 2-acetylaminofluorene and cyclphosphamide on immunoglobulin gene expression = 2-Acetylaminofluorene과 Cyclophosphamide에 의한 항체 형성 유전자 발현에 관한 연구

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The metabolism and subsequent immunosuppressive effects of 2-acetylaminofluorene (AAF), cyclophosphamide (CP) were investigated in mouse splenocytes, in mixed cultures of mouse splenocytes and rat hepatocytes (coculture). This immunosuppression was identified by the quantitative analysis of x mRNA using immunoglobulin x constant region specific human genomic DNA probes in hybridization. Also, to determine whether new protein synthesis is required for immunosuppression, we treated mouse splenocytes with LPS and AAF (or CP) in the presence of cycloheximide (CHX, 10㎍/ml). Direct addition of AAF into culture medium resulted in the decrease of x mRNA, but the metabolism of AAF was not required in suppression of x mRNA and CP, vice versa. That is, CP was activated by hepatocytes to an immunosuppressive form that caused the suppression of the in vitro antibody response to the B cell-dependent antigen, lipopolysaccharide (LPS). But the dose-response of CP showed that only 1mM, not 0.01mM and 0.1mM CP, decreased x mRNA. It seems that immunosuppression by CP is caused by the cytotoxicity rather than the inhibition of transcription of the immunoglobulin gene. Also, we found that a simultaneous treatment of AAF and LPS largely decreased x mRNA level. Therefore, it seems that AAF may block the early activation step in antibody gene expression activation mechanism by LPS. We identified that new protein synthesis is not required for immunosuppression by CP, AAF and unstimulated spleen cells also contain relatively high steady-state ievel of immunoglobulin mRNA when cultured for 48 hr, but they do not secrete antibody at a high rate until stimulated by LPS. In conclusion, the decreased level of immunoglobulin x mRNA can be interpreted as a failure of cell activation and splicing of primary transcripts.
Advisors
Yang, Kyu-Hwan양규환
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1992
Identifier
60364/325007 / 000901356
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ vi, 49 p. ]

URI
http://hdl.handle.net/10203/27748
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60364&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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