DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyunwoo | ko |
dc.contributor.author | Seo, Yeong-Hyeon | ko |
dc.contributor.author | Jeon, Jaehun | ko |
dc.contributor.author | Jeong, Ki-Hun | ko |
dc.date.accessioned | 2020-11-16T02:55:06Z | - |
dc.date.available | 2020-11-16T02:55:06Z | - |
dc.date.created | 2020-11-03 | - |
dc.date.created | 2020-11-03 | - |
dc.date.created | 2020-11-03 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | BIOMEDICAL OPTICS EXPRESS, v.11, no.10, pp.5575 - 5585 | - |
dc.identifier.issn | 2156-7085 | - |
dc.identifier.uri | http://hdl.handle.net/10203/277286 | - |
dc.description.abstract | High-resolution fluorescent microscopic imaging techniques are in high demand to observe detailed structures or dynamic mechanisms of biological samples. Structured illumination microscopy (SIM) has grabbed much attention in super-resolution imaging due to simple configuration, high compatibility with common fluorescent molecules, and fast image acquisition. Here, we report Lissajous scanning SIM (LS-SIM) by using a high fill-factor Lissajous scanning micromirror and laser beam modulation. The LS-SIM was realized by a Lissajous scanned structured illumination module, relay optics, and a conventional fluorescent microscope. The micromirror comprises an inner mirror and an outer frame, which are scanned at pseudo-resonance with electrostatic actuation. The biaxial scanning frequencies are selected by the frequency selection rule for high fill-factor (> 80%) Lissajous scanning. Structured illumination (SI) was then realized by modulating the intensity of a laser beam at the least common multiple (LCM) of the scanning frequencies. A compact Lissajous scanned SI module containing a fiber-optic collimator and Lissajous micromirror has been fully packaged and coupled with relay optics and a fiber-based diode pumped solid state (DPSS) laser including acousto-optic-modulator (ACM). Various structured images were obtained by shifting the phase and orientation of the illumination patterns and finally mounted with a conventional fluorescent microscope. The LS-SIM has experimentally demonstrated high-resolution fluorescent microscopic imaging of reference targets and human lung cancer cell PC-9 cells. The LS-SIM exhibits the observable region in spatial frequency space over 2x, the line-edge sharpness over 1.5x, and the peak-to-valley (P-V) ratio over 2x, compared to widefield fluorescent microscopy. This method can provide a new route for advanced high-resolution fluorescent microscopic imaging. | - |
dc.language | English | - |
dc.publisher | OPTICAL SOC AMER | - |
dc.title | Lissajous scanning structured illumination microscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000577572500015 | - |
dc.identifier.scopusid | 2-s2.0-85092300076 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 5575 | - |
dc.citation.endingpage | 5585 | - |
dc.citation.publicationname | BIOMEDICAL OPTICS EXPRESS | - |
dc.identifier.doi | 10.1364/BOE.404220 | - |
dc.contributor.localauthor | Jeong, Ki-Hun | - |
dc.contributor.nonIdAuthor | Kim, Hyunwoo | - |
dc.contributor.nonIdAuthor | Jeon, Jaehun | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
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