Functional characterization of the trans-synaptic adhesion between NGL-3 and LAR at excitatory synapses = 흥분성 시냅스에서 NGL-3와 LAR 결합과 그 기능에 대한 연구

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Synaptic cell adhesion molecules regulate multiple steps of synapse formation and maturation. The great diversity of neuronal synapses predicts the presence of a large number of adhesion molecules that control synapse formation through trans-synaptic and heterophilic adhesion. I identify a novel trans-synaptic interaction between NGL-3, a PSD-95-interacting postsynaptic adhesion molecule, and LAR, a receptor protein tyrosine phosphatase. The leucine-rich repeat (LRR) domain of NGL-3, containing nine LRRs, interacts with the first two fibronectin III (FNIII) domains of LAR to induce bidirectional synapse formation. Moreover, Gln 96 in the first LRR motif of NGL-3 is critical for LAR binding and induction of presynaptic differentiation. Neuronal overexpression of NGL-3 increases presynaptic contacts on dendrites of transfected neurons. Direct aggregation of NGL-3 on dendrites induces coclustering of excitatory postsynaptic proteins. Knockdown of NGL-3 reduces the number of excitatory synapses, but not inhibitory synapses. These results suggest that trans-interaction between NGL-3 and LAR is involved in excitatory synaptic differentiation.
Advisors
Kim, Eun-Joonresearcher김은준researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
455359/325007  / 020057360
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2010.08, [ viii, 84 p. ]

Keywords

adhesion; synapse; LAR; NGL; synaptogenesis; 시냅스형성; 접착; 시냅스; 라; 엔지엘

URI
http://hdl.handle.net/10203/27708
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=455359&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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