DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Doyeon | ko |
dc.contributor.author | Jang, Sangwon | ko |
dc.contributor.author | Kim, Jeongah | ko |
dc.contributor.author | Park, Inah | ko |
dc.contributor.author | Ku, Kyojin | ko |
dc.contributor.author | Choi, Mijung | ko |
dc.contributor.author | Lee, Sukwon | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Son, Gi Hoon | ko |
dc.contributor.author | Choe, Han Kyoung | ko |
dc.contributor.author | Kim, Kyungjin | ko |
dc.date.accessioned | 2020-10-29T06:55:07Z | - |
dc.date.available | 2020-10-29T06:55:07Z | - |
dc.date.created | 2020-10-26 | - |
dc.date.created | 2020-10-26 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | NEUROENDOCRINOLOGY, v.110, no.11-12, pp.1010 - 1027 | - |
dc.identifier.issn | 0028-3835 | - |
dc.identifier.uri | http://hdl.handle.net/10203/277028 | - |
dc.description.abstract | Introduction:Synchronous and pulsatile neural activation of kisspeptin neurons in the arcuate nucleus (ARN) are important components of the gonadotropin-releasing hormone pulse generator, the final common pathway for central regulation of mammalian reproduction. However, whether ARN kisspeptin neurons can intrinsically generate self-sustained synchronous oscillations from the early neonatal period and how they are regulated remain unclear.Objective:This study aimed to examine the endogenous rhythmicity of ARN kisspeptin neurons and its neural regulation using a neonatal organotypic slice culture model.Methods:We monitored calcium (Ca2+) dynamics in real-time from individual ARN kisspeptin neurons in neonatal organotypic explant cultures ofKiss1-IRES-Cre mice transduced with genetically encoded Ca2+ indicators. Pharmacological approaches were employed to determine the regulations of kisspeptin neuron-specific Ca2+ oscillations. A chemogenetic approach was utilized to assess the contribution of ARN kisspeptin neurons to the population dynamics.Results:ARN kisspeptin neurons in neonatal organotypic cultures exhibited a robust synchronized Ca2+ oscillation with a period of approximately 3 min. Kisspeptin neuron-specific Ca2+ oscillations were dependent on voltage-gated sodium channels and regulated by endoplasmic reticulum-dependent Ca2+ homeostasis. Chemogenetic inhibition of kisspeptin neurons abolished synchronous Ca2+ oscillations, but the autocrine actions of the neuropeptides were marginally effective. Finally, neonatal ARN kisspeptin neurons were regulated by N-methyl-D-aspartate and gamma-aminobutyric acid receptor-mediated neurotransmission.Conclusion:These data demonstrate that ARN kisspeptin neurons in organotypic cultures can generate synchronized and self-sustained Ca2+ oscillations. These oscillations controlled by multiple regulators within the ARN are a novel ultradian rhythm generator that is active during the early neonatal period. | - |
dc.language | English | - |
dc.publisher | KARGER | - |
dc.title | Kisspeptin Neuron-Specific and Self-Sustained Calcium Oscillation in the Hypothalamic Arcuate Nucleus of Neonatal Mice: Regulatory Factors of its Synchronization | - |
dc.type | Article | - |
dc.identifier.wosid | 000576042600013 | - |
dc.identifier.scopusid | 2-s2.0-85092680519 | - |
dc.type.rims | ART | - |
dc.citation.volume | 110 | - |
dc.citation.issue | 11-12 | - |
dc.citation.beginningpage | 1010 | - |
dc.citation.endingpage | 1027 | - |
dc.citation.publicationname | NEUROENDOCRINOLOGY | - |
dc.identifier.doi | 10.1159/000505922 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Kim, Doyeon | - |
dc.contributor.nonIdAuthor | Jang, Sangwon | - |
dc.contributor.nonIdAuthor | Kim, Jeongah | - |
dc.contributor.nonIdAuthor | Park, Inah | - |
dc.contributor.nonIdAuthor | Ku, Kyojin | - |
dc.contributor.nonIdAuthor | Choi, Mijung | - |
dc.contributor.nonIdAuthor | Lee, Sukwon | - |
dc.contributor.nonIdAuthor | Son, Gi Hoon | - |
dc.contributor.nonIdAuthor | Choe, Han Kyoung | - |
dc.contributor.nonIdAuthor | Kim, Kyungjin | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Arcuate nucleus | - |
dc.subject.keywordAuthor | Kisspeptin | - |
dc.subject.keywordAuthor | Ca2+ oscillation | - |
dc.subject.keywordAuthor | Synchronization | - |
dc.subject.keywordAuthor | Neonate | - |
dc.subject.keywordPlus | NEUROKININ B | - |
dc.subject.keywordPlus | MEDIAN-EMINENCE | - |
dc.subject.keywordPlus | KISS1 NEURONS | - |
dc.subject.keywordPlus | OPTOGENETIC ACTIVATION | - |
dc.subject.keywordPlus | HORMONE SECRETION | - |
dc.subject.keywordPlus | CA2+ OSCILLATIONS | - |
dc.subject.keywordPlus | HIPPOCAMPAL CA1 | - |
dc.subject.keywordPlus | TETANUS TOXIN | - |
dc.subject.keywordPlus | GNRH NEURONS | - |
dc.subject.keywordPlus | FEMALE | - |
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