Bio-inspired surface modification for biomedical applications = 생체재료 응용을 위한 생체 모방형 표면 개질

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A metal stent that could elute plasmid DNA (pDNA) in a controlled manner for substrate-mediated gene transfection was fabricated by first coating with hyaluronic acid (HA) and subsequent deposition of pDNA. To create robust HA coating layer on stainless steel (SS316L) surface, HA was derivatized with dopamine which is a well-known adsorptive molecule involving mussel adhesion process. The HA-coated surface was verified by various analytical techniques and proved to be very hydrophilic and stable, also showing superior biocompatibility in terms of suppressed plasma protein adsorption. For surface loading of pDNA, cationic pDNA/polyethylenimine (PEI) polyplexes were prepared and ionically adsorbed onto the HA-coated SS316L surface. The adsorbed surface exhibited evenly distributed nano-granular topography while the polyplexes maintained the nano-particular morphology. The pDNA was released out in a controlled manner for a period of 10 days with maintaining structural integrity. The dual coated substrate with HA and pDNA/PEI polyplexes exhibited greatly enhanced gene transfection efficiency, when compared to both bare substrate adsorbed with the polyplexes and PEI/pDNA polyelectrolyte multilayers. Dually functionalized stent with HA and pDNA exhibited effective biocompatibility and gene transfection. Drug-eluting stent (DES) has been widely used for the effective treatment of obstructive coronary artery disease, preventing the occurrence of restenosis which was mainly caused by hyper-proliferation of smooth muscle cells. Here, we demonstrate the immobilization of heparin on the metal surface via a bio-inspired manner and subsequent build-up of therapeutic lay-by-layer multilayer assembled from paclitaxel (PTX) encapsulated poly(lactic-co-glycolic acid) grafted hyaluronic acid (HA-g-PLGA) micelles and poly-L-lysine (PLL). It was hypothesized that the heparinized metallic surface would create a non-thrombogenic environment, while controlled release of PTX from the...
Advisors
Park, Tae-Gwanresearcher박태관researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
418713/325007  / 020055822
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2010.2, [ x, 117 p. ]

Keywords

paclitaxel; gene delivery; drug eluting stent; surface modification; mesenchymal stem cell; 중간엽줄기세포; 파클리탁셀; 유전자 전달; 약물 용출성 스텐트; 표면 개질

URI
http://hdl.handle.net/10203/27687
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=418713&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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