DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Seo, Yeon-Soo | - |
dc.contributor.advisor | 서연수 | - |
dc.contributor.author | Kang, Min-Jung | - |
dc.contributor.author | 강민정 | - |
dc.date.accessioned | 2011-12-12T07:55:52Z | - |
dc.date.available | 2011-12-12T07:55:52Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=418659&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27683 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2010.2, [ xii, 120 p. ] | - |
dc.description.abstract | Flap endonuclease1 (Fen1) and Dna2 are two critical endonucleases that work conjointly for processing of Okazaki fragments in eukaryotes. Mus81-Mms4 is another structure-specific endonuclease to resolve stalled replication forks as well as toxic recombination intermediates. In this study, we found that Mus81-Mms4 suppresses the defect of dna2 mutations and that it has functional and physical interactions between Fen1. Mus81-Mms4 stimulated significantly Fen1 activity, accounting for its ability to restore the growth defect caused by dna2 mutation. Surprisingly, Fen1 dramatically stimulated the rate of Mus81-Mms4 cleavage of its all known substrates including regressed replication fork substrates. The ability of Fen1 to stimulate Mus81-Mms4 was independent of the catalytic function of Fen1. The mutual stimulation between Fen1 and Mus81-Mms4 occurred via a specific protein-protein interaction. Our $\It{in vitro}$ data indicate that Mus81-Mms4 and Fen1 are likely to act together during DNA replication via direct interaction. This is supported by the findings that $\It{fen1 mus81}$ or $\It{fen1 mms4}$ double mutants could not rescue of lethality. We discuss the significance of the physical interactions between Fen1, Dna2, and Mus81-Mms4 in context of DNA replication. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 유전자 재조합 | - |
dc.subject | DNA 복제 | - |
dc.subject | Recombination | - |
dc.subject | Fen1 | - |
dc.subject | DNA replication | - |
dc.subject | Mus81-Mms4 | - |
dc.subject | Dna2 | - |
dc.title | Involvement of flap endonuclease 1 in the cleavage of stalled replication forks | - |
dc.title.alternative | 정체된 DNA replication fork에서의 flap endonuclease 1의 역할 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 418659/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020037006 | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.localauthor | 서연수 | - |
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