A novel ratiometric fluorescence sensor NCS ((E)-N-(furan-2-ylmethyl)-2-(2 hydroxybenzylidene)hydrazine-lcarbothioamide) was synthesized by a combination of furfuryl isothiocyanate bearing hydrazine and salicylaldehyde. Sensor NCS exhibited a ratiometric fluorescent response toward In(3+)via thioamide tautomerization with blue-shifted emission from 484 to 444 nm in a near-perfect aqueous solution. NCS displayed a linear ratiometric relationship to micromolar concentrations (0-60 mu M) of In 311 and the detection limit was found to be 2.68 mu M. Moreover, sensor NCS exhibited an excellent selectivity for sensing In3+ in real samples as well as live cell and zebrafish. The sensing mechanism was demonstrated through UV-visible, fluorescent and H-1 NMR titrations, Job's plot, ESI-MS and theoretical calculations.