DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Hak-Sung | - |
dc.contributor.advisor | 김학성 | - |
dc.contributor.author | Oh, Eun-Keu | - |
dc.contributor.author | 오은규 | - |
dc.date.accessioned | 2011-12-12T07:55:35Z | - |
dc.date.available | 2011-12-12T07:55:35Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=310360&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27664 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2006.8, [ viii, 80 p. ] | - |
dc.description.abstract | Nano-particle, a main material of nano-technology in 1 ~ 100 nm of size scale has a wide applicability in various research fields including physics, chemistry, biology, and medical science etc. Precisely, it is useful for a reaction catalyst, a building block for nano-patterning use, a molecular tag for diagnosis, and a nano-sized factor vehicle etc. For this purpose, several kinds of nano-particles are being manufactured by using metals, nonmetals, and semi-conductors etc. The nano-particles are spotlighted in several applications including bio-engineering areas, because they have physico-chemical or optical characteristics peculiar according to the kinds or sizes of core metals. Therefore, a lot of researches have been accomplished actively in bio-engineering fields in order to analyze a cell image and a protein interaction etc. by using these characteristics. In this paper, I demonstrated the noble detection system for biomolecular interaction using fluorescence resonance energy transfer (FRET) between gold nanoparticle and quantum dot. At first by employing a streptavidin-biotin interaction as a model system, my sensing system enables us to assay the avidin concentration in sample solution. And another application, I showed this nanoparticles-based FRET system could be successfully used for the detection of protein glycosylation which is one of the most important post-translational modifications of proteins. In conventional methods, ELISA, microarray, electrochemical signal sensing, calorimetric sensing by aggregation between gold nanoparticles and the like have been attempted to detect inhibitors for bio-sensing that interferes a specific binding between bio-molecules. And there are several disadvantages like time-consuming or laborious process requested, labeling a specimen, amplifying a signal by an additional reaction, or needing a specimen in a large scale. However, my nanoparticles-based FRET system suggested noble detection method for a target molec... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Gold nanoparticle | - |
dc.subject | Quantum Dot | - |
dc.subject | FRET | - |
dc.subject | sensing | - |
dc.subject | 금나노입자 | - |
dc.subject | 양자점 | - |
dc.subject | 형광공명전이 | - |
dc.subject | 센싱 | - |
dc.subject | Gold nanoparticle | - |
dc.subject | Quantum Dot | - |
dc.subject | FRET | - |
dc.subject | sensing | - |
dc.subject | 금나노입자 | - |
dc.subject | 양자점 | - |
dc.subject | 형광공명전이 | - |
dc.subject | 센싱 | - |
dc.title | Development of nanoparticle-based sensing system for biomolecular interaction | - |
dc.title.alternative | 나노 입자를 기반으로 한 생체분자 상호작용 측정 시스템의 개발 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 310360/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020025185 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.localauthor | 김학성 | - |
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